Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus

Halavaty, Andrei S.; Rich, Rebecca L.; Chen, Chao; Joo, Jeong Chan; Minasov, George; Dubrovska, Ievgeniia; Winsor, James R.; Myszka, David G.; Duban, Mark; Shuvalova, Ludmilla; Yakunin, Alexander F.; Anderson, Wayne F.

doi:10.1107/S1399004715004228

Title: Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus

Journal Article · Sat Apr 25 00:00:00 EDT 2015 · Acta Crystallographica. Section D: Biological Crystallography (Online)

DOI:https://doi.org/10.1107/S1399004715004228· OSTI ID:1233329

Halavaty, Andrei S. ^[1]; Rich, Rebecca L. ^[2]; Chen, Chao ^[3]; Joo, Jeong Chan ^[3]; Minasov, George ^[1]; Dubrovska, Ievgeniia ^[1]; Winsor, James R. ^[1]; Myszka, David G. ^[2]; Duban, Mark ^[4]; Shuvalova, Ludmilla ^[1]; Yakunin, Alexander F. ^[3]; Anderson, Wayne F. ^[1]

Northwestern Univ., Chicago, IL (United States). Dept. of Biochemistry and Molecular Genetics; Center for Structural Genomics of Infectious Diseases (CSGID), Chicago, IL (United States)
Biosensor Tools LLC, Salt Lake City, UT (United States)
Univ. of Toronto, ON (Canada). Dept. of Chemical Engineering and Applied Chemistry
Center for Structural Genomics of Infectious Diseases (CSGID), Chicago, IL (United States)

When exposed to high osmolarity, methicillin-resistant Staphylococcus aureus (MRSA) restores its growth and establishes a new steady state by accumulating the osmoprotectant metabolite betaine. Effective osmoregulation has also been implicated in the acquirement of a profound antibiotic resistance by MRSA. Betaine can be obtained from the bacterial habitat or produced intracellularly from choline via the toxic betaine aldehyde (BA) employing the choline dehydrogenase and betaine aldehyde dehydrogenase (BADH) enzymes. Here, it is shown that the putative betaine aldehyde dehydrogenase SACOL2628 from the early MRSA isolate COL ( SaBADH) utilizes betaine aldehyde as the primary substrate and nicotinamide adenine dinucleotide (NAD⁺) as the cofactor. Surface plasmon resonance experiments revealed that the affinity of NAD⁺, NADH and BA for SaBADH is affected by temperature, pH and buffer composition. Finally, five crystal structures of the wild type and three structures of the Gly234Ser mutant of SaBADH in the apo and holo forms provide details of the molecular mechanisms of activity and substrate specificity/inhibition of this enzyme.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357; 085P1000817; HHSN272200700058C; HHSN272201200026C

OSTI ID:: 1233329

Journal Information:: Acta Crystallographica. Section D: Biological Crystallography (Online), Vol. 71, Issue 5; ISSN 1399-0047

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 15 works

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Studying NAD(P)H cofactor-binding to alcohol dehydrogenases through global analysis of circular dichroism spectra Marolt, Marija; Lüdeke, Steffen Physical Chemistry Chemical Physics, Vol. 21, Issue 4 https://doi.org/10.1039/c8cp04869j	journal	January 2019
Structural insights into betaine aldehyde dehydrogenase (BADH2) from Oryza sativa explored by modeling and simulations Baicharoen, Apisara; Vijayan, Ranjit; Pongprayoon, Prapasiri Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-31204-z	journal	August 2018
An expansin-like protein expands forage cell walls and synergistically increases hydrolysis, digestibility and fermentation of livestock feeds by fibrolytic enzymes Pech-Cervantes, Andres A.; Ogunade, Ibukun M.; Jiang, Yun PLOS ONE, Vol. 14, Issue 11 https://doi.org/10.1371/journal.pone.0224381	journal	November 2019

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structural genomics
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