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Title: MetaBAT

Abstract

Assembling individual genomes from shotgun metagenomic sequences derived from complex microbial communities is so far one of the most challenging problems in bioinformatics.As it is impractical to directly assemble full-length genomes, a first step that groups contigs from the same organisms, called metagenome binning, has been developed to provide insights of individual organisms. However, current binning methods perform poorly in the context of large complex community, and as a result they fail to recover many novel genomes. To overcome this limitation, we developed integrated software, called MetaBAT, which automatically forms hundreds of individual genome bins from metagenome contigs. Probabilistic models of abundance and tetranucleotide frequency were trained by extensive empirical studies and integrated to decide the membership of contigs iteratively. To test the performance of MetaBAT, we applied MetaBAT to both synthetic and several large-scale real world metagenome datasets. By using two independent metrics, we demonstrate that in all the data sets tested MetaBAT achieves good sensitivity (16~87%) and very high specificity (56~99%) in forming genome bins. Further analyses of the novel genomes recovered from the human gut microbiome suggest a subset of these genomes are potentially associated with pathological conditions. In conclusion, we believe MetaBAT is a powerful tool

Authors:
; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1231763
Report Number(s):
METABAT; 003006MLTPL00
2014-075
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Software
Software Revision:
00
Software Package Number:
003006
Software Package Contents:
All Distribution will be handled by Lawrence Berkeley National Laboratory.
Software CPU:
MLTPL
Open Source:
No
Source Code Available:
Yes
Country of Publication:
United States

Citation Formats

Kang, Dongwan, Egan, Rob, Froula, Jeff, and Wang, Zhnog. MetaBAT. Computer software. Vers. 00. USDOE. 1 Apr. 2014. Web.
Kang, Dongwan, Egan, Rob, Froula, Jeff, & Wang, Zhnog. (2014, April 1). MetaBAT (Version 00) [Computer software].
Kang, Dongwan, Egan, Rob, Froula, Jeff, and Wang, Zhnog. MetaBAT. Computer software. Version 00. April 1, 2014.
@misc{osti_1231763,
title = {MetaBAT, Version 00},
author = {Kang, Dongwan and Egan, Rob and Froula, Jeff and Wang, Zhnog},
abstractNote = {Assembling individual genomes from shotgun metagenomic sequences derived from complex microbial communities is so far one of the most challenging problems in bioinformatics.As it is impractical to directly assemble full-length genomes, a first step that groups contigs from the same organisms, called metagenome binning, has been developed to provide insights of individual organisms. However, current binning methods perform poorly in the context of large complex community, and as a result they fail to recover many novel genomes. To overcome this limitation, we developed integrated software, called MetaBAT, which automatically forms hundreds of individual genome bins from metagenome contigs. Probabilistic models of abundance and tetranucleotide frequency were trained by extensive empirical studies and integrated to decide the membership of contigs iteratively. To test the performance of MetaBAT, we applied MetaBAT to both synthetic and several large-scale real world metagenome datasets. By using two independent metrics, we demonstrate that in all the data sets tested MetaBAT achieves good sensitivity (16~87%) and very high specificity (56~99%) in forming genome bins. Further analyses of the novel genomes recovered from the human gut microbiome suggest a subset of these genomes are potentially associated with pathological conditions. In conclusion, we believe MetaBAT is a powerful tool},
doi = {},
url = {https://www.osti.gov/biblio/1231763}, year = {Tue Apr 01 00:00:00 EDT 2014},
month = {Tue Apr 01 00:00:00 EDT 2014},
note =
}

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