Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics

Geng, Tao; Bredeweg, Erin L.; Szymanski, Craig J.; Liu, Bingwen; Baker, Scott E.; Orr, Galya; Evans, James E.; Kelly, Ryan T.

doi:10.1038/srep16111

Title: Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics

Journal Article · Wed Nov 04 00:00:00 EST 2015 · Scientific Reports

DOI:https://doi.org/10.1038/srep16111· OSTI ID:1226407

Geng, Tao ^[1]; Bredeweg, Erin L. ^[1]; Szymanski, Craig J. ^[1]; Liu, Bingwen ^[1]; Baker, Scott E. ^[1]; Orr, Galya ^[1]; Evans, James E. ^[1]; Kelly, Ryan T. ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Here, interrogating polarized growth is technologically challenging due to extensive cellular branching and uncontrollable environmental conditions in conventional assays. Here we present a robust and high-performance microfluidic system that enables observations of polarized growth with enhanced temporal and spatial control over prolonged periods. The system has built-in tunability and versatility to accommodate a variety of science applications requiring precisely controlled environments. Using the model filamentous fungus, Neurospora crassa, this microfluidic system enabled direct visualization and analysis of cellular heterogeneity in a clonal fungal cell population, nuclear distribution and dynamics at the subhyphal level, and quantitative dynamics of gene expression with single hyphal compartment resolution in response to carbon source starvation and exchange experiments. Although the microfluidic device is demonstrated on filamentous fungi, our technology is immediately extensible to a wide array of other biosystems that exhibit similar polarized cell growth with applications ranging from bioenergy production to human health.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; 66382

OSTI ID:: 1226407

Report Number(s):: PNNL-SA-109268; 48672; KP1704020

Journal Information:: Scientific Reports, Vol. 5, Issue 16111; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 22 works

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Title: Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics

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