skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Phosphoketolase pathway engineering for carbon-efficient biocatalysis

Abstract

Recent advances in metabolic engineering have facilitated the development of microbial biocatalysts capable of producing an array of bio-products, ranging from fuels to drug molecules. These bio-products are commonly generated through an acetyl-CoA intermediate, which serves as a key precursor in the biological conversion of carbon substrates. Moreover, conventional biocatalytic upgrading strategies proceeding through this route are limited by low carbon efficiencies, in large part due to carbon losses associated with pyruvate decarboxylation to acetyl-CoA. Bypass of pyruvate decarboxylation offers a means to dramatically enhance carbon yields and, in turn, bioprocess economics. Here, we discuss recent advances and prospects for employing the phosphoketolase pathway for direct biosynthesis of acetyl-CoA from carbon substrates, and phosphoketolase-based metabolic engineering strategies for carbon efficient biocatalysis.

Authors:
; ;
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1225341
Report Number(s):
NREL/JA-5100-65107
Journal ID: ISSN 0958-1669
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Journal Name:
Current Opinion in Biotechnology
Additional Journal Information:
Journal Volume: 36; Journal Issue: C; Related Information: Current Opinion in Biotechnology; Journal ID: ISSN 0958-1669
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; microbial biocatalysts; biocatalysis; phosphoketolase; biosynthesis

Citation Formats

Henard, Calvin Andrew, Freed, Emily Frances, and Guarnieri, Michael Thomas. Phosphoketolase pathway engineering for carbon-efficient biocatalysis. United States: N. p., 2015. Web. doi:10.1016/j.copbio.2015.08.018.
Henard, Calvin Andrew, Freed, Emily Frances, & Guarnieri, Michael Thomas. Phosphoketolase pathway engineering for carbon-efficient biocatalysis. United States. https://doi.org/10.1016/j.copbio.2015.08.018
Henard, Calvin Andrew, Freed, Emily Frances, and Guarnieri, Michael Thomas. 2015. "Phosphoketolase pathway engineering for carbon-efficient biocatalysis". United States. https://doi.org/10.1016/j.copbio.2015.08.018.
@article{osti_1225341,
title = {Phosphoketolase pathway engineering for carbon-efficient biocatalysis},
author = {Henard, Calvin Andrew and Freed, Emily Frances and Guarnieri, Michael Thomas},
abstractNote = {Recent advances in metabolic engineering have facilitated the development of microbial biocatalysts capable of producing an array of bio-products, ranging from fuels to drug molecules. These bio-products are commonly generated through an acetyl-CoA intermediate, which serves as a key precursor in the biological conversion of carbon substrates. Moreover, conventional biocatalytic upgrading strategies proceeding through this route are limited by low carbon efficiencies, in large part due to carbon losses associated with pyruvate decarboxylation to acetyl-CoA. Bypass of pyruvate decarboxylation offers a means to dramatically enhance carbon yields and, in turn, bioprocess economics. Here, we discuss recent advances and prospects for employing the phosphoketolase pathway for direct biosynthesis of acetyl-CoA from carbon substrates, and phosphoketolase-based metabolic engineering strategies for carbon efficient biocatalysis.},
doi = {10.1016/j.copbio.2015.08.018},
url = {https://www.osti.gov/biblio/1225341}, journal = {Current Opinion in Biotechnology},
issn = {0958-1669},
number = C,
volume = 36,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 2015},
month = {Tue Dec 01 00:00:00 EST 2015}
}

Works referenced in this record:

Phosphoketolase, a Neglected Enzyme of Microbial Carbohydrate Metabolism
journal, June 2002


Bacterial and Eukaryotic Phosphoketolases: Phylogeny, Distribution and Evolution
journal, January 2010

  • Sánchez, Borja; Zúñiga, Manuel; González-Candelas, Fernando
  • Journal of Molecular Microbiology and Biotechnology, Vol. 18, Issue 1
  • https://doi.org/10.1159/000274310

Unique Sugar Metabolic Pathways of Bifidobacteria
journal, December 2010


Acetate kinase: not just a bacterial enzyme
journal, June 2006


Evidence, through C13-labelling analysis, of phosphoketolase activity in fungi
journal, July 2007


Innovative metabolic pathway design for efficient l-glutamate production by suppressing CO2 emission
journal, March 2007


Improved polyhydroxybutyrate production by Saccharomyces cerevisiae through the use of the phosphoketolase pathway
journal, March 2013


Induction of xylulose-5-phosphate phosphoketolase in a variety of yeasts grown ond-xylose: the key to efficient xylose metabolism
journal, September 1984


Properties of a pentulose-5-phosphate phosphoketolase from yeasts grown on xylose
journal, July 1985


Metabolic Engineering of a Phosphoketolase Pathway for Pentose Catabolism in Saccharomyces cerevisiae
journal, May 2004


Studies of the Production of Fungal Polyketides in Aspergillus nidulans by Using Systems Biology Tools
journal, January 2009


Synthetic non-oxidative glycolysis enables complete carbon conservation
journal, September 2013


Building carbon–carbon bonds using a biocatalytic methanol condensation cycle
journal, October 2014


Metabolic engineering in methanotrophic bacteria
journal, May 2015


Rubisco activity and regulation as targets for crop improvement
journal, November 2012


Envisioning the Bioconversion of Methane to Liquid Fuels
journal, February 2014


Rethinking biological activation of methane and conversion to liquid fuels
journal, April 2014


Highly efficient methane biocatalysis revealed in a methanotrophic bacterium
journal, December 2013


Acetyl phosphate and the activation of two-component response regulators.
journal, December 1994


Is acetyl phosphate a global signal in Escherichia coli?
journal, January 1993


Acetyl Coenzyme A: A Central Metabolite and Second Messenger
journal, June 2015


Works referencing / citing this record:

Growth-coupled evolution of phosphoketolase to improve l-glutamate production by Corynebacterium glutamicum
journal, August 2019


Artificial pathway emergence in central metabolism from three recursive phosphoketolase reactions
journal, November 2018


Holistic bioengineering: rewiring central metabolism for enhanced bioproduction
journal, November 2017


Engineering cytoplasmic acetyl-CoA synthesis decouples lipid production from nitrogen starvation in the oleaginous yeast Rhodosporidium azoricum
journal, November 2019


Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design
journal, March 2019


Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae
journal, November 2016


Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design
journal, March 2019


Engineering cytoplasmic acetyl-CoA synthesis decouples lipid production from nitrogen starvation in the oleaginous yeast Rhodosporidium azoricum
journal, November 2019


Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae
journal, November 2016