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Title: Phosphoketolase pathway engineering for carbon-efficient biocatalysis

Recent advances in metabolic engineering have facilitated the development of microbial biocatalysts capable of producing an array of bio-products, ranging from fuels to drug molecules. These bio-products are commonly generated through an acetyl-CoA intermediate, which serves as a key precursor in the biological conversion of carbon substrates. Moreover, conventional biocatalytic upgrading strategies proceeding through this route are limited by low carbon efficiencies, in large part due to carbon losses associated with pyruvate decarboxylation to acetyl-CoA. Bypass of pyruvate decarboxylation offers a means to dramatically enhance carbon yields and, in turn, bioprocess economics. Here, we discuss recent advances and prospects for employing the phosphoketolase pathway for direct biosynthesis of acetyl-CoA from carbon substrates, and phosphoketolase-based metabolic engineering strategies for carbon efficient biocatalysis.
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Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 0958-1669
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Current Opinion in Biotechnology; Journal Volume: 36; Journal Issue: C; Related Information: Current Opinion in Biotechnology
Research Org:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Country of Publication:
United States
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES microbial biocatalysts; biocatalysis; phosphoketolase; biosynthesis