Phosphoketolase pathway engineering for carbon-efficient biocatalysis
Abstract
Recent advances in metabolic engineering have facilitated the development of microbial biocatalysts capable of producing an array of bio-products, ranging from fuels to drug molecules. These bio-products are commonly generated through an acetyl-CoA intermediate, which serves as a key precursor in the biological conversion of carbon substrates. Moreover, conventional biocatalytic upgrading strategies proceeding through this route are limited by low carbon efficiencies, in large part due to carbon losses associated with pyruvate decarboxylation to acetyl-CoA. Bypass of pyruvate decarboxylation offers a means to dramatically enhance carbon yields and, in turn, bioprocess economics. Here, we discuss recent advances and prospects for employing the phosphoketolase pathway for direct biosynthesis of acetyl-CoA from carbon substrates, and phosphoketolase-based metabolic engineering strategies for carbon efficient biocatalysis.
- Authors:
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1225341
- Report Number(s):
- NREL/JA-5100-65107
Journal ID: ISSN 0958-1669
- DOE Contract Number:
- AC36-08GO28308
- Resource Type:
- Journal Article
- Journal Name:
- Current Opinion in Biotechnology
- Additional Journal Information:
- Journal Volume: 36; Journal Issue: C; Related Information: Current Opinion in Biotechnology; Journal ID: ISSN 0958-1669
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; microbial biocatalysts; biocatalysis; phosphoketolase; biosynthesis
Citation Formats
Henard, Calvin Andrew, Freed, Emily Frances, and Guarnieri, Michael Thomas. Phosphoketolase pathway engineering for carbon-efficient biocatalysis. United States: N. p., 2015.
Web. doi:10.1016/j.copbio.2015.08.018.
Henard, Calvin Andrew, Freed, Emily Frances, & Guarnieri, Michael Thomas. Phosphoketolase pathway engineering for carbon-efficient biocatalysis. United States. https://doi.org/10.1016/j.copbio.2015.08.018
Henard, Calvin Andrew, Freed, Emily Frances, and Guarnieri, Michael Thomas. 2015.
"Phosphoketolase pathway engineering for carbon-efficient biocatalysis". United States. https://doi.org/10.1016/j.copbio.2015.08.018.
@article{osti_1225341,
title = {Phosphoketolase pathway engineering for carbon-efficient biocatalysis},
author = {Henard, Calvin Andrew and Freed, Emily Frances and Guarnieri, Michael Thomas},
abstractNote = {Recent advances in metabolic engineering have facilitated the development of microbial biocatalysts capable of producing an array of bio-products, ranging from fuels to drug molecules. These bio-products are commonly generated through an acetyl-CoA intermediate, which serves as a key precursor in the biological conversion of carbon substrates. Moreover, conventional biocatalytic upgrading strategies proceeding through this route are limited by low carbon efficiencies, in large part due to carbon losses associated with pyruvate decarboxylation to acetyl-CoA. Bypass of pyruvate decarboxylation offers a means to dramatically enhance carbon yields and, in turn, bioprocess economics. Here, we discuss recent advances and prospects for employing the phosphoketolase pathway for direct biosynthesis of acetyl-CoA from carbon substrates, and phosphoketolase-based metabolic engineering strategies for carbon efficient biocatalysis.},
doi = {10.1016/j.copbio.2015.08.018},
url = {https://www.osti.gov/biblio/1225341},
journal = {Current Opinion in Biotechnology},
issn = {0958-1669},
number = C,
volume = 36,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 2015},
month = {Tue Dec 01 00:00:00 EST 2015}
}
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