Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy

Moerner, W. E. (William E. ).

doi:10.1103/RevModPhys.87.1183

Title: Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy

Authors:: Moerner, W. E. (William E. ).

Publication Date:: Wed Oct 21 00:00:00 EDT 2015

Sponsoring Org.:: USDOE

OSTI Identifier:: 1224141

Grant/Contract Number:: FG02-07ER15892

Resource Type:: Journal Article: Publisher's Accepted Manuscript

Journal Name:: Reviews of Modern Physics

Additional Journal Information:: Journal Name: Reviews of Modern Physics Journal Volume: 87 Journal Issue: 4; Journal ID: ISSN 0034-6861

Publisher:: American Physical Society

Country of Publication:: United States

Language:: English

Citation Formats


                    Moerner, W. E. Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy.  United States: N. p., 2015. 
        Web.  doi:10.1103/RevModPhys.87.1183.

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                    Moerner, W. E. Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy.  United States.  https://doi.org/10.1103/RevModPhys.87.1183

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                    Moerner, W. E. 2015.  
        "Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy".  United States.  https://doi.org/10.1103/RevModPhys.87.1183.

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@article{osti_1224141,

  title        = {Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy},

  author       = {Moerner, W. E.},

  abstractNote = {},

  doi          = {10.1103/RevModPhys.87.1183},

  url          = {https://www.osti.gov/biblio/1224141},
  journal      = {Reviews of Modern Physics},

  issn         = {0034-6861},

  number       = 4,

  volume       = 87,

  place        = {United States},

  year         = {Wed Oct 21 00:00:00 EDT 2015},

  month        = {Wed Oct 21 00:00:00 EDT 2015}

}

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Hess, Samuel T.; Girirajan, Thanu P. K.; Mason, Michael D.
Biophysical Journal, Vol. 91, Issue 11
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Fluorescence spectroscopy and spectral diffusion of single impurity molecules in a crystal
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Ambrose, W. P.; Moerner, W. E.
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From spectral holeburning memory to spatial-spectral microwave signal processing
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Babbitt, Wm Randall; Barber, Zeb W.; Bekker, Scott H.
Laser Physics, Vol. 24, Issue 9
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The Role of Molecular Dipole Orientation in Single-Molecule Fluorescence Microscopy and Implications for Super-Resolution Imaging
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Backlund, Mikael P.; Lew, Matthew D.; Backer, Adam S.
ChemPhysChem, Vol. 15, Issue 4
https://doi.org/10.1002/cphc.201300880

A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
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Patterson, G. H.
Science, Vol. 297, Issue 5588
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Illuminating Single Molecules in Condensed Matter
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Single Molecule Detection in Solution
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Zander, Christoph; Enderlein, Jörg; Keller, Richard A.
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Single-molecule fluorescence detection: autocorrelation criterion and experimental realization with phycoerythrin.
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Peck, K.; Stryer, L.; Glazer, A. N.
Proceedings of the National Academy of Sciences, Vol. 86, Issue 11
https://doi.org/10.1073/pnas.86.11.4087

Small-Molecule Labeling of Live Cell Surfaces for Three-Dimensional Super-Resolution Microscopy
journal, August 2014

Lee, Marissa K.; Rai, Prabin; Williams, Jarrod
Journal of the American Chemical Society, Vol. 136, Issue 40
https://doi.org/10.1021/ja508028h

Cholesterol Depletion Induces Solid-like Regions in the Plasma Membrane
journal, February 2006

Nishimura, Stefanie Y.; Vrljic, Marija; Klein, Lawrence O.
Biophysical Journal, Vol. 90, Issue 3
https://doi.org/10.1529/biophysj.105.070524

Fluorescence Spectroscopy of Single Biomolecules
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Weiss, S.
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Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP
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Biteen, Julie S.; Thompson, Michael A.; Tselentis, Nicole K.
Nature Methods, Vol. 5, Issue 11
https://doi.org/10.1038/nmeth.1258

Regulated Fast Nucleocytoplasmic Shuttling Observed by Reversible Protein Highlighting
journal, November 2004

Ando, R.
Science, Vol. 306, Issue 5700
https://doi.org/10.1126/science.1102506

Large single-molecule fluorescence enhancements produced by a bowtie nanoantenna
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Kinkhabwala, Anika; Yu, Zongfu; Fan, Shanhui
Nature Photonics, Vol. 3, Issue 11, p. 654-657
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Shapes of Inhomogeneously Broadened Resonance Lines in Solids
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Stoneham, A. M.
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Neher, Erwin; Sakmann, Bert
Nature, Vol. 260, Issue 5554
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Single pentacene molecules detected by fluorescence excitation in a p -terphenyl crystal
journal, November 1990

Orrit, M.; Bernard, J.
Physical Review Letters, Vol. 65, Issue 21
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Citation Formats

Multicolor Fluorescence Nanoscopy in Fixed and Living Cells by Exciting Conventional Fluorophores with a Single Wavelength journal, October 2010

Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. SHORT COMMUNICATION journal, May 2000

High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells journal, December 2008

Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. journal, June 1996

The Green Fluorescent Protein journal, June 1998

SPDM: light microscopy with single-molecule resolution at the nanoscale journal, September 2008

On/off blinking and switching behaviour of single molecules of green fluorescent protein journal, July 1997

Nanometer-localized multiple single-molecule fluorescence microscopy journal, July 2004

Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels journal, November 1996

An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein journal, September 2002

Scanning tunneling microscopy—from birth to adolescence journal, July 1987

Far-field fluorescence microscopy beyond the diffraction limit journal, January 1999

Super-resolution Microscopy Approaches for Live Cell Imaging journal, October 2014

Single Molecules Observed by Near-Field Scanning Optical Microscopy journal, November 1993

Imaging and Time-Resolved Spectroscopy of Single Molecules at an Interface journal, April 1996

Proposed method for molecular optical imaging journal, January 1995

Probing individual molecules with confocal fluorescence microscopy journal, November 1994

Microscopy beyond the diffraction limit using actively controlled single molecules journal, April 2012

Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) journal, August 2006

The Helical MreB Cytoskeleton in Escherichia coli MC1000/pLE7 Is an Artifact of the N-Terminal Yellow Fluorescent Protein Tag journal, December 2012

Precise Nanometer Localization Analysis for Individual Fluorescent Probes journal, May 2002

Fluorescence Correlation Spectroscopy and Its Potential for Intracellular Applications journal, January 2001

Detection of single fluorescent molecules journal, November 1990

Detection and spectroscopy of single pentacene molecules in a p ‐terphenyl crystal by means of fluorescence excitation journal, November 1991

Sub-Diffraction Imaging of Huntingtin Protein Aggregates by Fluorescence Blink-Microscopy and Atomic Force Microscopy journal, July 2011

Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms journal, May 2013

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells journal, December 2010

Methods of single-molecule fluorescence spectroscopy and microscopy journal, August 2003

Superresolution microscopy for microbiology: Superresolution microscopy for microbiology journal, October 2012

Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects journal, January 2011

Probing Single Molecule Dynamics journal, July 1994

Magnetic resonance of a single molecular spin journal, May 1993

Ultrahigh-resolution multicolor colocalization of single fluorescent probes journal, August 2000

Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores journal, November 2010

Optical detection and spectroscopy of single molecules in a solid journal, May 1989

Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung journal, December 1873

Fluorescent Saxitoxins for Live Cell Imaging of Single Voltage-Gated Sodium Ion Channels beyond the Optical Diffraction Limit journal, July 2012

Optimal Point Spread Function Design for 3D Imaging journal, September 2014

Single molecule detection and photochemistry on a surface using near-field optical excitation journal, January 1994

Fluorescence transient and optical free induction decay spectroscopy of pentacene in mixed crystals at 2 K. Determination of intersystem crossing and internal conversion rates journal, June 1979

Single molecule polarization spectroscopy: pentacene in p-terphenyl journal, November 1996

Tracking single molecules at work in living cells journal, June 2014

A bisected pupil for studying single-molecule orientational dynamics and its application to three-dimensional super-resolution microscopy journal, May 2014

The effect of fine structure appearance in laser-excited fluorescence spectra of organic compounds in solid solutions journal, October 1972

Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution journal, April 1995

Spectral diffusion of single molecule fluorescence: A probe of low-frequency localized excitations in disordered crystals journal, December 1993

New directions in single-molecule imaging and analysis journal, July 2007

High-Resolution Colocalization of Single Dye Molecules by Fluorescence Lifetime Imaging Microscopy journal, July 2002

Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy journal, February 2008

Fluorescence correlation spectroscopy. II. An experimental realization journal, January 1974

Fluorescence correlation spectroscopy. I. Conceptual basis and theory journal, January 1974

Tracking kinesin-driven movements with nanometre-scale precision journal, February 1988

Extending Microscopic Resolution with Single-Molecule Imaging and Active Control journal, June 2012

Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer. journal, August 1996

A spindle-like apparatus guides bacterial chromosome segregation journal, July 2010

A practical guide to single-molecule FRET journal, May 2008

Optimized localization analysis for single-molecule tracking and super-resolution microscopy journal, April 2010

Fluorescence correlation spectroscopy as a probe of molecular dynamics journal, February 1976

Persistent Spectral Hole-Burning: Science and Applications book, January 1988

Atomic Force Microscope journal, March 1986

Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization journal, June 2003

Thermodynamic Fluctuations in a Reacting System—Measurement by Fluorescence Correlation Spectroscopy journal, September 1972

Photon antibunching in the fluorescence of a single dye molecule trapped in a solid journal, September 1992

Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus journal, October 2011

Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples journal, May 2008

Single molecules of the bacterial actin MreB undergo directed treadmilling motion in Caulobacter crescentus journal, July 2006

Probing Single Biomolecules in Solution Using the Anti-Brownian Electrokinetic (ABEL) Trap journal, March 2012

Single-molecule high-resolution imaging with photobleaching journal, April 2004

Rotational brownian motion and fluorescence intensify fluctuations journal, June 1974

Fluorescence microscopy of single molecules journal, January 1994

Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function journal, February 2009

Imaging Intracellular Fluorescent Proteins at Nanometer Resolution journal, September 2006

Examining Nanoenvironments in Solids on the Scale of a Single, Isolated Impurity Molecule journal, July 1994

Optical Probing of Single Molecules of Terrylene in a Shpol'kii Matrix: A Two-State Single-Molecule Switch journal, July 1994

3-Dimensional super-resolution by spectrally selective imaging journal, July 1998

Position measurement with a resolution and noise‐limited instrument journal, June 1986

Frequency modulation (FM) spectroscopy: Theory of lineshapes and signal-to-noise analysis journal, November 1983

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy journal, January 1994

Multicolor Fluorescence Nanoscopy in Fixed and Living Cells by Exciting Conventional Fluorophores with a Single Wavelength
journal, October 2010

Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. SHORT COMMUNICATION
journal, May 2000

High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells
journal, December 2008

Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor.
journal, June 1996

The Green Fluorescent Protein
journal, June 1998

SPDM: light microscopy with single-molecule resolution at the nanoscale
journal, September 2008

On/off blinking and switching behaviour of single molecules of green fluorescent protein
journal, July 1997

Nanometer-localized multiple single-molecule fluorescence microscopy
journal, July 2004

Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels
journal, November 1996

An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
journal, September 2002

Scanning tunneling microscopy—from birth to adolescence
journal, July 1987

Far-field fluorescence microscopy beyond the diffraction limit
journal, January 1999

Super-resolution Microscopy Approaches for Live Cell Imaging
journal, October 2014

Single Molecules Observed by Near-Field Scanning Optical Microscopy
journal, November 1993

Imaging and Time-Resolved Spectroscopy of Single Molecules at an Interface
journal, April 1996

Proposed method for molecular optical imaging
journal, January 1995

Probing individual molecules with confocal fluorescence microscopy
journal, November 1994

Microscopy beyond the diffraction limit using actively controlled single molecules
journal, April 2012

Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
journal, August 2006

The Helical MreB Cytoskeleton in Escherichia coli MC1000/pLE7 Is an Artifact of the N-Terminal Yellow Fluorescent Protein Tag
journal, December 2012

Precise Nanometer Localization Analysis for Individual Fluorescent Probes
journal, May 2002

Fluorescence Correlation Spectroscopy and Its Potential for Intracellular Applications
journal, January 2001

Detection of single fluorescent molecules
journal, November 1990

Detection and spectroscopy of single pentacene molecules in a p ‐terphenyl crystal by means of fluorescence excitation
journal, November 1991

Sub-Diffraction Imaging of Huntingtin Protein Aggregates by Fluorescence Blink-Microscopy and Atomic Force Microscopy
journal, July 2011

Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms
journal, May 2013

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells
journal, December 2010

Methods of single-molecule fluorescence spectroscopy and microscopy
journal, August 2003

Superresolution microscopy for microbiology: Superresolution microscopy for microbiology
journal, October 2012

Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects
journal, January 2011

Probing Single Molecule Dynamics
journal, July 1994

Magnetic resonance of a single molecular spin
journal, May 1993

Ultrahigh-resolution multicolor colocalization of single fluorescent probes
journal, August 2000

Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores
journal, November 2010

Optical detection and spectroscopy of single molecules in a solid
journal, May 1989

Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung
journal, December 1873

Fluorescent Saxitoxins for Live Cell Imaging of Single Voltage-Gated Sodium Ion Channels beyond the Optical Diffraction Limit
journal, July 2012

Optimal Point Spread Function Design for 3D Imaging
journal, September 2014

Single molecule detection and photochemistry on a surface using near-field optical excitation
journal, January 1994

Fluorescence transient and optical free induction decay spectroscopy of pentacene in mixed crystals at 2 K. Determination of intersystem crossing and internal conversion rates
journal, June 1979

Single molecule polarization spectroscopy: pentacene in p-terphenyl
journal, November 1996

Tracking single molecules at work in living cells
journal, June 2014

A bisected pupil for studying single-molecule orientational dynamics and its application to three-dimensional super-resolution microscopy
journal, May 2014

The effect of fine structure appearance in laser-excited fluorescence spectra of organic compounds in solid solutions
journal, October 1972

Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution
journal, April 1995

Spectral diffusion of single molecule fluorescence: A probe of low-frequency localized excitations in disordered crystals
journal, December 1993

New directions in single-molecule imaging and analysis
journal, July 2007

High-Resolution Colocalization of Single Dye Molecules by Fluorescence Lifetime Imaging Microscopy
journal, July 2002

Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
journal, February 2008

Fluorescence correlation spectroscopy. II. An experimental realization
journal, January 1974

Fluorescence correlation spectroscopy. I. Conceptual basis and theory
journal, January 1974

Tracking kinesin-driven movements with nanometre-scale precision
journal, February 1988

Extending Microscopic Resolution with Single-Molecule Imaging and Active Control
journal, June 2012

Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer.
journal, August 1996

A spindle-like apparatus guides bacterial chromosome segregation
journal, July 2010

A practical guide to single-molecule FRET
journal, May 2008

Optimized localization analysis for single-molecule tracking and super-resolution microscopy
journal, April 2010

Fluorescence correlation spectroscopy as a probe of molecular dynamics
journal, February 1976

Persistent Spectral Hole-Burning: Science and Applications
book, January 1988

Atomic Force Microscope
journal, March 1986

Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
journal, June 2003

Thermodynamic Fluctuations in a Reacting System—Measurement by Fluorescence Correlation Spectroscopy
journal, September 1972

Photon antibunching in the fluorescence of a single dye molecule trapped in a solid
journal, September 1992

Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus
journal, October 2011

Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples
journal, May 2008

Single molecules of the bacterial actin MreB undergo directed treadmilling motion in Caulobacter crescentus
journal, July 2006

Probing Single Biomolecules in Solution Using the Anti-Brownian Electrokinetic (ABEL) Trap
journal, March 2012

Single-molecule high-resolution imaging with photobleaching
journal, April 2004

Rotational brownian motion and fluorescence intensify fluctuations
journal, June 1974

Fluorescence microscopy of single molecules
journal, January 1994

Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function
journal, February 2009

Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
journal, September 2006

Examining Nanoenvironments in Solids on the Scale of a Single, Isolated Impurity Molecule
journal, July 1994

Optical Probing of Single Molecules of Terrylene in a Shpol'kii Matrix: A Two-State Single-Molecule Switch
journal, July 1994

3-Dimensional super-resolution by spectrally selective imaging
journal, July 1998

Position measurement with a resolution and noise‐limited instrument
journal, June 1986

Frequency modulation (FM) spectroscopy: Theory of lineshapes and signal-to-noise analysis
journal, November 1983

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
journal, January 1994

Imaging of single molecule diffusion.
journal, April 1996

Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane
journal, October 2010