Characterization of novel sorghum brown midrib mutants from an EMS-mutagenized population

Sattler, Scott E.; Saballos, Ana; Xin, Zhanguo; Funnell-Harris, Deanna L.; Vermerris, Wilfred; Pedersen, Jeffrey F.

doi:10.1534/g3.114.014001

Title: Characterization of novel sorghum brown midrib mutants from an EMS-mutagenized population

Journal Article · Tue Sep 02 00:00:00 EDT 2014 · G3

DOI:https://doi.org/10.1534/g3.114.014001· OSTI ID:1222462

Sattler, Scott E. ^[1]; Saballos, Ana ^[2]; Xin, Zhanguo ^[3]; Funnell-Harris, Deanna L. ^[1]; Vermerris, Wilfred ^[2]; Pedersen, Jeffrey F. ^[1]

USDA-ARS, Lincoln, NE (United States); Univ. of Nebraska-Lincoln, Lincoln, NE (United States)
Univ. of Florida, Gainesville, FL (United States)
USDA-ARS, Lubbock, TX (United States)

Reducing lignin concentration in lignocellulosic biomass can increase forage digestibility for ruminant livestock and saccharification yields of biomass for bioenergy. In sorghum (Sorghum bicolor (L.) Moench) and several other C4 grasses, brown midrib (bmr) mutants have been shown to reduce lignin concentration. Putative bmr mutants isolated from an EMS-mutagenized population were characterized and classified based on their leaf midrib phenotype and allelism tests with the previously described sorghum bmr mutants bmr2, bmr6, and bmr12. These tests resulted in the identification of additional alleles of bmr2, bmr6,and bmr12, and, in addition, six bmr mutants were identified that were not allelic to these previously described loci. Further allelism testing among these six bmr mutants showed that they represented four novel bmr loci. Based on this study, the number of bmr loci uncovered in sorghum has doubled. The impact of these lines on agronomic traits and lignocellulosic composition was assessed in a 2-yr field study. Most of the identified bmr lines showed reduced lignin concentration of their biomass relative to wild-type (WT). Effects of the six new bmr mutants on enzymatic saccharification of lignocellulosic materials were determined, but the amount of glucose released from the stover was similar to WT in all cases. Like bmr2, bmr6, and bmr12, these mutants may affect monolignol biosynthesis and may be useful for bioenergy and forage improvement when stacked together or in combination with the three previously described bmr alleles.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FG02-07ER64458

OSTI ID:: 1222462

Journal Information:: G3, Vol. 4, Issue 11; ISSN 2160-1836

Publisher:: Genetics Society of AmericaCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 47 works

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Title: Characterization of novel sorghum brown midrib mutants from an EMS-mutagenized population

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