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Title: Multifunctional cellulase and hemicellulase

Abstract

A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.

Inventors:
; ;
Publication Date:
Research Org.:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1221980
Patent Number(s):
9,145,551
Application Number:
14/030,290
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
DOE Contract Number:  
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Sep 18
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Fox, Brian G., Takasuka, Taichi, and Bianchetti, Christopher M. Multifunctional cellulase and hemicellulase. United States: N. p., 2015. Web.
Fox, Brian G., Takasuka, Taichi, & Bianchetti, Christopher M. Multifunctional cellulase and hemicellulase. United States.
Fox, Brian G., Takasuka, Taichi, and Bianchetti, Christopher M. 2015. "Multifunctional cellulase and hemicellulase". United States. https://www.osti.gov/servlets/purl/1221980.
@article{osti_1221980,
title = {Multifunctional cellulase and hemicellulase},
author = {Fox, Brian G. and Takasuka, Taichi and Bianchetti, Christopher M.},
abstractNote = {A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.},
doi = {},
url = {https://www.osti.gov/biblio/1221980}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 29 00:00:00 EDT 2015},
month = {Tue Sep 29 00:00:00 EDT 2015}
}

Works referenced in this record:

Promoters and proteins from Clostridium thermocellum and uses thereof
patent-application, May 2006


Action of Designer Cellulosomes on Homogeneous Versus Complex Substrates : CONTROLLED INCORPORATION OF THREE DISTINCT ENZYMES INTO A DEFINED TRIFUNCTIONAL SCAFFOLDIN
journal, February 2005


Multiple display of catalytic modules on a protein scaffold: Nano-fabrication of enzyme particles
journal, September 2007


A major new component in the cellulosome of Clostridium thermocellum is a processive endo-β-1,4-glucanase producing cellotetraose
journal, August 2005


Exploring the Properties of Thermostable Clostridium thermocellum Cellulase CelE for the Purpose of Its Expression in Plants
journal, January 2001


Restructuring the Crystalline Cellulose Hydrogen Bond Network Enhances Its Depolymerization Rate
journal, July 2011


Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition
journal, January 2007


High-throughput Purification and Quality Assurance of Arabidopsis thaliana Proteins for Eukaryotic Structural Genomics
journal, September 2005


A combined approach to improving large-scale production of tobacco etch virus protease
journal, September 2007


Nanostructure‐Initiator Mass Spectrometry (NIMS) for the Analysis of Enzyme Activities
journal, June 2012


Cellulose-degrading bacteria associated with the invasive woodwasp Sirex noctilio
journal, March 2011


Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
journal, January 2013