Multifunctional cellulase and hemicellulase
Abstract
A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.
- Inventors:
- Publication Date:
- Research Org.:
- Wisconsin Alumni Research Foundation, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1221980
- Patent Number(s):
- 9,145,551
- Application Number:
- 14/030,290
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- DOE Contract Number:
- FC02-07ER64494
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2013 Sep 18
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Fox, Brian G., Takasuka, Taichi, and Bianchetti, Christopher M. Multifunctional cellulase and hemicellulase. United States: N. p., 2015.
Web.
Fox, Brian G., Takasuka, Taichi, & Bianchetti, Christopher M. Multifunctional cellulase and hemicellulase. United States.
Fox, Brian G., Takasuka, Taichi, and Bianchetti, Christopher M. 2015.
"Multifunctional cellulase and hemicellulase". United States. https://www.osti.gov/servlets/purl/1221980.
@article{osti_1221980,
title = {Multifunctional cellulase and hemicellulase},
author = {Fox, Brian G. and Takasuka, Taichi and Bianchetti, Christopher M.},
abstractNote = {A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.},
doi = {},
url = {https://www.osti.gov/biblio/1221980},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 29 00:00:00 EDT 2015},
month = {Tue Sep 29 00:00:00 EDT 2015}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Mycobacterium tuberculosis protein composition
patent, October 2009
- Fox, Brian G.; Chang, Yong
- US Patent Document 7,601,358
Xylanases, nucleic acids encoding them and methods for making and using them
patent, October 2011
- Weiner, David; Blum, David; Varvak, Alexander
- US Patent Document 8,043,839
Expression systems for functional membrane polypeptides
patent, January 2012
- Fox, Brian G.; Sobrado, Pablo
- US Patent Document 8,088,601
Carboxy terminal residues as predictors and regulators of protein stability
patent, September 2012
- Fox, Brian G.; Chang, Yong; Wesenberg, Gary A.
- US Patent Document 8,268,581
Promoters and proteins from Clostridium thermocellum and uses thereof
patent-application, May 2006
- Wu, J.H. David; Newcomb, Michael
- US Patent Application 11/271287; 20060105442
Cohesin-dockerin microarray: Diverse specificities between two complementary families of interacting protein modules
journal, March 2008
- Haimovitz, Rachel; Barak, Yoav; Morag, Ely
- PROTEOMICS, Vol. 8, Issue 5
Action of Designer Cellulosomes on Homogeneous Versus Complex Substrates : CONTROLLED INCORPORATION OF THREE DISTINCT ENZYMES INTO A DEFINED TRIFUNCTIONAL SCAFFOLDIN
journal, February 2005
- Fierobe, Henri-Pierre; Mingardon, Florence; Mechaly, Adva
- Journal of Biological Chemistry, Vol. 280, Issue 16
Multiple display of catalytic modules on a protein scaffold: Nano-fabrication of enzyme particles
journal, September 2007
- Heyman, Arnon; Barak, Yoav; Caspi, Jonathan
- Journal of Biotechnology, Vol. 131, Issue 4, p. 433-439
Characterization of a Cellulase Containing a Family 30 Carbohydrate-Binding Module (CBM) Derived from Clostridium thermocellum CelJ: Importance of the CBM to Cellulose Hydrolysis
journal, January 2003
- Arai, T.; Araki, R.; Tanaka, A.
- Journal of Bacteriology, Vol. 185, Issue 2
A major new component in the cellulosome of Clostridium thermocellum is a processive endo-β-1,4-glucanase producing cellotetraose
journal, August 2005
- Zverlov, Vladimir V.; Schantz, Nikolaus; Schwarz, Wolfgang H.
- FEMS Microbiology Letters, Vol. 249, Issue 2
Exploring the Properties of Thermostable Clostridium thermocellum Cellulase CelE for the Purpose of Its Expression in Plants
journal, January 2001
- Abdeev, R. M.; Goldenkova, I. V.; Musiychuk, K. A.
- Biochemistry (Moscow), Vol. 66, Issue 7, p. 808-813
Restructuring the Crystalline Cellulose Hydrogen Bond Network Enhances Its Depolymerization Rate
journal, July 2011
- Chundawat, Shishir P. S.; Bellesia, Giovanni; Uppugundla, Nirmal
- Journal of the American Chemical Society, Vol. 133, Issue 29
Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition
journal, January 2007
- Blommel, Paul G.; Becker, Katie J.; Duvnjak, Petar
- Biotechnology Progress, Vol. 23, Issue 3
High-throughput Purification and Quality Assurance of Arabidopsis thaliana Proteins for Eukaryotic Structural Genomics
journal, September 2005
- Jeon, Won Bae; Aceti, David J.; Bingman, Craig A.
- Journal of Structural and Functional Genomics, Vol. 6, Issue 2-3
A combined approach to improving large-scale production of tobacco etch virus protease
journal, September 2007
- Blommel, Paul G.; Fox, Brian G.
- Protein Expression and Purification, Vol. 55, Issue 1, p. 53-68
Nanostructure‐Initiator Mass Spectrometry (NIMS) for the Analysis of Enzyme Activities
journal, June 2012
- Reindl, Wolfgang; Deng, Kai; Cheng, Xiaoliang
- Current Protocols in Chemical Biology, Vol. 4, Issue 2
Cellulose-degrading bacteria associated with the invasive woodwasp Sirex noctilio
journal, March 2011
- Adams, Aaron S.; Jordan, Michelle S.; Adams, Sandye M.
- The ISME Journal, Vol. 5, Issue 8
Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
journal, January 2013
- Takasuka, Taichi E.; Book, Adam J.; Lewin, Gina R.
- Scientific Reports, Vol. 3, Issue 1