Ionic switch controls the DNA state in phage λ

Li, Dong; Liu, Ting; Zuo, Xiaobing; Li, Tao; Qiu, Xiangyun; Evilevitch, Alex

doi:10.1093/nar/gkv611

Title: Ionic switch controls the DNA state in phage λ

Journal Article · Fri Jun 19 00:00:00 EDT 2015 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkv611· OSTI ID:1221840

Li, Dong ^[1]; Liu, Ting ^[1]; Zuo, Xiaobing ^[2]; Li, Tao ^[2]; Qiu, Xiangyun ^[3]; Evilevitch, Alex ^[4]

Carnegie Mellon Univ., Pittsburgh, PA (United States). Physics Dept.
Argonne National Lab., Argonne, IL (United States). Advanced Photon Source, X-ray Science Div.
George Washington Univ., Washington, DC (United States). Dept. of Physics.
Carnegie Mellon Univ., Pittsburgh, PA (United States). Physics Dept.; Lund Univ., Lund (Sweden). Dept. of Biochemistry and Structural Biology.

We have recently found that DNA packaged in phage λ undergoes a disordering transition triggered by temperature, which results in increased genome mobility. This solid-to-fluid like DNA transition markedly increases the number of infectious λ particles facilitating infection. However, the structural transition strongly depends on temperature and ionic conditions in the surrounding medium. Using titration microcalorimetry combined with solution X-ray scattering, we mapped both energetic and structural changes associated with transition of the encapsidated λ-DNA. Packaged DNA needs to reach a critical stress level in order for transition to occur. We varied the stress on DNA in the capsid by changing the temperature, packaged DNA length and ionic conditions. We found striking evidence that the intracapsid DNA transition is ‘switched on’ at the ionic conditions mimicking those in vivo and also at the physiologic temperature of infection at 37°C. This ion regulated on-off switch of packaged DNA mobility in turn affects viral replication. The results suggest a remarkable adaptation of phage λ to the environment of its host bacteria in the human gut. The metastable DNA state in the capsid provides a new paradigm for the physical evolution of viruses.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1221840

Journal Information:: Nucleic Acids Research, Vol. 43, Issue 13; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 11 works

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