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Title: Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling

The plant hormone jasmonate plays crucial roles in regulating plant responses to herbivorous insects and microbial pathogens and is an important regulator of plant growth and development. Key mediators of jasmonate signalling include MYC transcription factors, which are repressed by jasmonate ZIM-domain (JAZ) transcriptional repressors in the resting state. In the presence of active jasmonate, JAZ proteins function as jasmonate co-receptors by forming a hormone-dependent complex with COI1, the F-box subunit of an SCF-type ubiquitin E3 ligase. The hormone-dependent formation of the COI1–JAZ co-receptor complex leads to ubiquitination and proteasome-dependent degradation of JAZ repressors and release of MYC proteins from transcriptional repression. The mechanism by which JAZ proteins repress MYC transcription factors and how JAZ proteins switch between the repressor function in the absence of hormone and the co-receptor function in the presence of hormone remain enigmatic. In this paper, we show that Arabidopsis MYC3 undergoes pronounced conformational changes when bound to the conserved Jas motif of the JAZ9 repressor. The Jas motif, previously shown to bind to hormone as a partly unwound helix, forms a complete α-helix that displaces the amino (N)-terminal helix of MYC3 and becomes an integral part of the MYC N-terminal fold. In this position, themore » Jas helix competitively inhibits MYC3 interaction with the MED25 subunit of the transcriptional Mediator complex. Finally, our structural and functional studies elucidate a dynamic molecular switch mechanism that governs the repression and activation of a major plant hormone pathway.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [3] ;  [7] ;  [8] ;  [5] ;  [9] ;  [10] ;  [3] ;  [11]
  1. Van Andel Research Inst. (VARI), Grand Rapids, MI (United States). Lab. of Structural Biology and Biochemistry. Lab. of Structural Sciences; Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Lab.; Nanjing Agricultural Univ. (China). College of Plant Protection
  2. Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Lab.; Western Michigan Univ., Kalamazoo, MI (United States). Dept. of Biological Sciences
  3. Van Andel Research Inst. (VARI), Grand Rapids, MI (United States). Lab. of Structural Biology and Biochemistry. Lab. of Structural Sciences
  4. Michigan State Univ., East Lansing, MI (United States). Dept. of Plant Biology. DOE Plant Research Lab.
  5. Scripps Research Inst., Jupiter, FL (United States). Dept. of Molecular Therapeutics. Translational Research Inst.
  6. Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Lab.
  7. Van Andel Research Inst. (VARI), Grand Rapids, MI (United States). Lab. of Structural Biology and Biochemistry. Lab. of Structural Sciences; Zhejiang Sci-Tech Univ., Hangzhou (China). College of Life Sciences
  8. Northwestern Univ., Argonne, IL (United States). Synchrotron Research Center. Life Sciences Collaborative Access Team. Dept. of Molecular Pharmacology and Biological Chemistry
  9. Nanjing Agricultural Univ. (China). College of Plant Protection
  10. Van Andel Research Inst. (VARI), Grand Rapids, MI (United States). Lab. of Structural Biology and Biochemistry. Lab. of Structural Sciences; Chinese Academy of Sciences (CAS), Shanghai (China). Shanghai Inst. for Biological Sciences. Shanghai Inst. of Materia Medica. Center for Structure and Function of Drug Targets. VARI-SIMM Center. Key Lab. of Receptor Research
  11. Michigan State Univ., East Lansing, MI (United States). Dept. of Plant Biology. DOE Plant Research Lab. Howard Hughes Medical Inst.
Publication Date:
OSTI Identifier:
1221449
Grant/Contract Number:
FG02-91ER20021; AC02-06CH11357; R01 GM102545; R01AI060761; GBMF3037; 085P1000817
Type:
Accepted Manuscript
Journal Name:
Nature (London)
Additional Journal Information:
Journal Name: Nature (London); Journal Volume: 525; Journal Issue: 7568; Journal ID: ISSN 0028-0836
Publisher:
Nature Publishing Group
Research Org:
Van Andel Research Inst. (VARI), Grand Rapids, MI (United States); Michigan State Univ., East Lansing, MI (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Inst. of Health (NIH) (United States); Gordon and Betty Moore Foundation (United States); Van Andel Research Inst. (United States); Michigan Economic Development Corporation (United States); Michigan Technology Tri-Corridor (United States); Chinese Scholarship Council
Contributing Orgs:
Nanjing Agricultural Univ. (China); Western Michigan Univ., Kalamazoo, MI (United States); Scripps Research Inst., Jupiter, FL (United States); Zhejiang Sci-Tech Univ., Hangzhou (China); Northwestern Univ., Argonne, IL (United States); Chinese Academy of Sciences (CAS), Shanghai (China)
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; Plant hormones; Structural biology; Plant signalling