Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius

Cabañes, F. Javier; Sanseverino, Walter; Castellá, Gemma; Bragulat, M. Rosa; Cigliano, Riccardo Aiese; Sánchez, Armand

doi:10.1038/srep09086

Title: Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius

Journal Article · Fri Mar 13 00:00:00 EDT 2015 · Scientific Reports

DOI:https://doi.org/10.1038/srep09086· OSTI ID:1215424

Cabañes, F. Javier ^[1]; Sanseverino, Walter ^[2]; Castellá, Gemma ^[1]; Bragulat, M. Rosa ^[1]; Cigliano, Riccardo Aiese ^[2]; Sánchez, Armand ^[1]

Univ. Autònoma de Barcelona, Bellaterra, Catalonia (Spain)
Sequentia Biotech SL, Barcelona, Catalonia (Spain)

In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis.

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Research Organization:: Universitat Autònoma de Barcelona, Bellaterra, Catalonia (Spain)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: Joint Genome Institute, Walnut Creek, CA (United States)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1215424

Journal Information:: Scientific Reports, Vol. 5; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 13 works

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Genomic diversity in ochratoxigenic and non ochratoxigenic strains of Aspergillus carbonarius Castellá, Gemma; Bragulat, M. Rosa; Puig, Laura Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-23802-8	journal	April 2018
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