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Title: Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape During In Vitro Culture

Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. We observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Also, quantitative data were usedmore » to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 3',5'-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism..« less
 [1] ;  [1] ;  [2] ;  [3] ;  [1] ;  [4] ;  [5] ;  [3] ;  [6] ;  [7] ;  [2] ;  [2]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Leibniz Inst. of Plant Genetics and Crop Plant Research, Gatersleben (Germany)
  3. Univ. of Hannover (Germany)
  4. Brookhaven National Lab. (BNL), Upton, NY (United States); Indian Inst. of Advanced Research Koba, Gujarat (India)
  5. Monash Univ., Melbourne, VIC (Australia)
  6. Monash Univ., Melbourne, VIC (Australia); Univ. Halle-Wittenberg, Melbourne (Australia)
  7. Bayer CropScience (Belgium)
Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 1040-4651; R&D Project: BO-133; 650201010
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Plant Cell; Journal Volume: 168; Journal Issue: 3
American Society of Plant Biologists
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES Brassica napus; 13C-MFA flux analysis; seed development; storage metabolism; systems biology