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Title: Reagent-free and portable detection of Bacillus anthracis spores using a microfluidic incubator and smartphone microscope

Rapid, cost-effective bacterial detection systems are needed to respond to potential biothreat events. Here we report the use of smartphone-based microscopy in combination with a simple microfluidic incubation device to detect 5000 Bacillus anthracis spores in 3 hours. This field-deployable approach is compatible with real-time PCR for secondary confirmation.
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
1213000
Report Number(s):
PNNL-SA-109030
47618
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Analyst, 140(18):6269-6276
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
B. anthracis; spores; smartphone microscope; microfluidic incubation chamber; Environmental Molecular Sciences Laboratory