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Title: Production of carrier-peptide conjugates using chemically reactive unnatural amino acids

Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.
Authors:
;
Publication Date:
OSTI Identifier:
1210885
Report Number(s):
9,109,022
14/065,111
DOE Contract Number:
FG03-00ER46051
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Oct 28
Research Org:
The Scripps Research Institute, La Jolla, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES