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Title: Electron-caputure dissociation and ion mobility mass spectrometry for characterization of the hemoglobin protein assembly

Journal Article · · Protein Sci.
DOI:https://doi.org/10.1002/pro.2712· OSTI ID:1210822

Research Organization:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
DOE Contract Number:
SC0001035
OSTI ID:
1210822
Journal Information:
Protein Sci., Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK
Country of Publication:
United States
Language:
English

References (58)

The molecular sociology of the cell journal December 2007
Integrating mass spectrometry of intact protein complexes into structural proteomics journal May 2012
X-ray crystallography at the heart of life science journal October 2011
Mass Spectrometry Methods for Studying Structure and Dynamics of Biological Macromolecules journal December 2013
Mass spectrometry-based methods to study protein architecture and dynamics: MS-Based Methods to Study Protein Architecture and Dynamics journal March 2013
Unraveling the dynamics of protein interactions with quantitative mass spectrometry journal March 2011
Hydrogen Exchange Mass Spectrometry: Are We Out of the Quicksand? journal April 2012
Hydroxyl Radical-Mediated Modification of Proteins as Probes for Structural Proteomics journal August 2007
Painting proteins with covalent labels: What’s in the picture? journal June 2009
Probing protein structure by amino acid-specific covalent labeling and mass spectrometry journal September 2009
Studying noncovalent protein complexes by electrospray ionization mass spectrometry journal January 1997
Twenty Years of Gas Phase Structural Biology journal September 2013
Native mass spectrometry: a bridge between interactomics and structural biology journal October 2008
Peeling back the layers of complexity journal October 2011
Studying 18 MDa Virus Assemblies with Native Mass Spectrometry journal February 2013
Mass spectrometry of intact membrane protein complexes journal March 2013
Ion mobility–mass spectrometry for structural proteomics journal February 2012
Collisional activation of protein complexes: Picking up the pieces journal March 2009
Principles of electron capture and transfer dissociation mass spectrometry applied to peptide and protein structure analysis journal January 2013
Top-Down ESI-ECD-FT-ICR Mass Spectrometry Localizes Noncovalent Protein-Ligand Binding Sites journal November 2006
Native Electrospray and Electron-Capture Dissociation in FTICR Mass Spectrometry Provide Top-Down Sequencing of a Protein Component in an Intact Protein Assembly journal December 2010
Native Electrospray and Electron-Capture Dissociation FTICR Mass Spectrometry for Top-Down Studies of Protein Assemblies journal July 2011
The emergence of protein complexes: quaternary structure, dynamics and allostery journal May 2012
Electron Transfer Dissociation Mass Spectrometry of Hemoglobin on Clinical Samples journal August 2012
Top-Down Proteomics and Direct Surface Sampling of Neonatal Dried Blood Spots: Diagnosis of Unknown Hemoglobin Variants journal September 2012
Top-down analysis of small plasma proteins using an LTQ-Orbitrap. Potential for mass spectrometry-based clinical assays for transthyretin and hemoglobin journal March 2011
Top-Down and Bottom-Up Identification of Proteins by Liquid Extraction Surface Analysis Mass Spectrometry of Healthy and Diseased Human Liver Tissue journal September 2014
Collision Cross Sections of Proteins and Their Complexes: A Calibration Framework and Database for Gas-Phase Structural Biology journal November 2010
T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis journal January 2010
Cation-Induced Stabilization of Protein Complexes in the Gas Phase: Mechanistic Insights From Hemoglobin Dissociation Studies journal January 2014
Comparative Analysis of Oxy-Hemoglobin and Aquomet-Hemoglobin by Hydrogen/Deuterium Exchange Mass Spectrometry journal May 2013
An Electrostatic Charge Partitioning Model for the Dissociation of Protein Complexes in the Gas Phase journal July 2011
Mass Spectra and Ion Collision Cross Sections of Hemoglobin journal January 2011
Determinants of Gas-Phase Disassembly Behavior in Homodimeric Protein Complexes with Related Yet Divergent Structures journal May 2011
Gas-phase H/D exchange and collision cross sections of hemoglobin monomers, dimers, and tetramers journal March 2009
Probing hemoglobin structure by means of traveling-wave ion mobility mass spectrometry journal April 2009
Noncovalent Protein Tetramers and Pentamers with “ n ” Charges Yield Monomers with n /4 and n /5 Charges journal February 2009
Folding and assembly of hemoglobin monitored by electrospray mass spectrometry using an on-line dialysis system journal January 2007
Assembly of Hemoglobin from Denatured Monomeric Subunits: Heme Ligation Effects and Off-Pathway Intermediates Studied by Electrospray Mass Spectrometry journal February 2013
Subunit Disassembly and Unfolding Kinetics of Hemoglobin Studied by Time-Resolved Electrospray Mass Spectrometry journal November 2004
Ion mobility–mass spectrometry analysis of large protein complexes journal June 2008
Ion mobility mass spectrometry of proteins and proteinassemblies journal January 2010
Gas-phase metalloprotein complexes interrogated by ion mobility-mass spectrometry journal June 2009
Evidence for Macromolecular Protein Rings in the Absence of Bulk Water journal December 2005
Ion Mobility–Mass Spectrometry Reveals Long-Lived, Unfolded Intermediates in the Dissociation of Protein Complexes journal October 2007
Ion Mobility-Mass Spectrometry Reveals the Influence of Subunit Packing and Charge on the Dissociation of Multiprotein Complexes journal December 2010
Native Top-Down Electrospray Ionization-Mass Spectrometry of 158 kDa Protein Complex by High-Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry journal December 2013
Revealing Ligand Binding Sites and Quantifying Subunit Variants of Noncovalent Protein Complexes in a Single Native Top-Down FTICR MS Experiment journal June 2014
ETD Allows for Native Surface Mapping of a 150 kDa Noncovalent Complex on a Commercial Q-TWIMS-TOF Instrument journal January 2014
Extending Top-Down Mass Spectrometry to Proteins with Masses Greater Than 200 Kilodaltons journal October 2006
Consecutive Ion Activation for Top Down Mass Spectrometry:  Improved Protein Sequencing by Nozzle−Skimmer Dissociation journal September 2005
Effects of Protein–Ligand Interactions on Hydrogen/Deuterium Exchange Kinetics: Canonical and Noncanonical Scenarios journal June 2014
A brief history of hemoglobins: plant, animal, protist, and bacteria. journal June 1996
Electrostatic Stabilization of a Native Protein Structure in the Gas Phase journal November 2010
Top-down mass spectrometry: Recent developments, applications and perspectives journal January 2011
Do Charge State Signatures Guarantee Protein Conformations? journal May 2012
Characterization of Native Protein Complexes Using Ultraviolet Photodissociation Mass Spectrometry journal September 2014
Fundamentals of Trapped Ion Mobility Spectrometry journal October 2014