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Title: Functional Characteristics of Spirilloxanthin and Keto-Bearing Analogues in Light-Harvesting LH2 Complexes from Rhodobacter sphaeroides with a Genetically Modified Carotenoid Synthesis Pathway

Journal Article · · Biochim. Biophys. Acta, Bioenerg.

Research Organization:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
DOE Contract Number:
SC0001035
OSTI ID:
1210515
Journal Information:
Biochim. Biophys. Acta, Bioenerg., Vol. 1847; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK
Country of Publication:
United States
Language:
English

References (64)

Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria journal April 1995
Apoprotein structure in the LH2 complex from Rhodopseudomonas acidophila strain 10050: modular assembly and protein pigment interactions 1 1Edited by R. Huber journal May 1997
The Structure and Thermal Motion of the B800–850 LH2 Complex from Rps.acidophila at 2.0Å Resolution and 100K: New Structural Features and Functionally Relevant Motions journal March 2003
The crystal structure of the light-harvesting complex II (B800–850) from Rhodospirillum molischianum journal May 1996
Conjugation-Length Dependence of the T 1 Lifetimes of Carotenoids Free in Solution and Incorporated into the LH2, LH1, RC, and RC-LH1 Complexes:  Possible Mechanisms of Triplet-Energy Dissipation journal February 2007
Photoprotection in a purple phototrophic bacterium mediated by oxygen-dependent alteration of carotenoid excited-state properties journal May 2012
Ultrafast Time-Resolved Carotenoid to-Bacteriochlorophyll Energy Transfer in LH2 Complexes from Photosynthetic Bacteria journal August 2008
Molecular Factors Controlling Photosynthetic Light Harvesting by Carotenoids journal August 2010
Optical and optically detected magnetic resonance investigation on purple photosynthetic bacterial antenna complexes journal May 1995
Carotenoids in Photosynthesis journal March 1996
Functional assembly of the foreign carotenoid lycopene into the photosynthetic apparatus of Rhodobacter sphaeroides, achieved by replacement of the native 3-step phytoene desaturase with its 4-step counterpart from Erwinia herbicola: Directed synthesis of lycopene in Rhodobacter journal April 2002
Dynamics of Energy Transfer from Lycopene to Bacteriochlorophyll in Genetically-Modified LH2 Complexes of Rhodobacter sphaeroides journal March 2002
Triplet excited state spectra and dynamics of carotenoids from the thermophilic purple photosynthetic bacterium Thermochromatium tepidum journal January 2011
Spectroscopic studies of two spectral variants of light-harvesting complex 2 (LH2) from the photosynthetic purple sulfur bacterium Allochromatium vinosum journal September 2012
Carotenoid diversity: a modular role for the phytoene desaturase step journal November 1998
Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway journal February 2015
The peripheral light-harvesting complexes from purple sulfur bacteria have different ‘ring’ sizes journal October 2008
Characterisation of the LH2 spectral variants produced by the photosynthetic purple sulphur bacterium Allochromatium vinosum journal November 2014
Global and target analysis of time-resolved spectra journal July 2004
Catalytic properties of the expressed acyclic carotenoid 2-ketolases from Rhodobacter capsulatus and Rubrivivax gelatinosus journal February 2009
Novel Activity of Rhodobacter sphaeroides Spheroidene Monooxygenase CrtA Expressed in Escherichia coli journal September 2010
Influence of Carotenoid Molecules on the Structure of the Bacteriochlorophyll Binding Site in Peripheral Light-Harvesting Proteins from Rhodobacter sphaeroides , journal June 2003
Carotenoid-Induced Cooperative Formation of Bacterial Photosynthetic LH1 Complex journal December 2004
Femtosecond Spectroscopy of Native and Carotenoidless Purple-Bacterial LH2 Clarifies Functions of Carotenoids journal June 2008
Resonance Raman spectroscopy of carotenoids and carotenoid-containing systems journal January 1985
Raman and infrared spectra of the all-trans, 7-cis, 9-cis, 13-cis and 15-cis isomers of β-carotene: Key bands distinguishing stretched or terminal-bent configurations form central-bent configurations journal February 1988
Electronic Absorption and Ground State Structure of Carotenoid Molecules journal January 2013
Transient Absorption from the 1Bu+ State of All-trans-β-carotene Newly Identified in the Near-infrared Region¶ journal January 2001
Ultrafast Dynamics of Carotenoid Excited States−From Solution to Natural and Artificial Systems journal April 2004
Dark excited states of carotenoids: Consensus and controversy journal July 2009
Near-Infrared Time-Resolved Study of the S 1 State Dynamics of the Carotenoid Spheroidene journal February 2001
Ultrafast Dynamics and Excited State Spectra of Open-Chain Carotenoids at Room and Low Temperatures journal May 2007
Ultrafast time-resolved absorption spectroscopy of geometric isomers of xanthophylls journal July 2010
Photophysics and dynamics of the lowest excited singlet state in long substituted polyenes with implications to the very long‐chain limit journal August 1995
Pump−Deplete−Probe Spectroscopy and the Puzzle of Carotenoid Dark States journal March 2004
An unusual pathway of excitation energy deactivation in carotenoids: Singlet-to-triplet conversion on an ultrafast timescale in a photosynthetic antenna journal February 2001
Femtosecond Time-Resolved Transient Absorption Spectroscopy of Xanthophylls journal November 2006
A Near-Infrared Transient Absorption Study of the Excited-State Dynamics of the Carotenoid Spirilloxanthin in Solution and in the LH1 Complex of Rhodospirillum rubrum journal October 2003
Ultrafast time-resolved absorption spectroscopy of geometric isomers of carotenoids journal February 2009
Sequential singlet internal conversion of 1Bu+→3Ag−→1Bu−→2Ag−→(1Ag− ground) in all-trans-spirilloxanthin revealed by two-dimensional sub-5-fs spectroscopy journal July 2004
Two different pathways of internal conversion in carotenoids depending on the length of the conjugated chain journal February 2003
Sub-picosecond excited-state dynamics of a carotenoid (spirilloxanthin) in the light-harvesting systems of Chromatium vinosum. journal September 1998
An alternative carotenoid-to-bacteriochlorophyll energy transfer pathway in photosynthetic light harvesting journal April 2002
Light Harvesting by Carotenoids Incorporated into the B850 Light-Harvesting Complex from Rhodobacter s phaeroides R-26.1:  Excited-State Relaxation, Ultrafast Triplet Formation, and Energy Transfer to Bacteriochlorophyll journal June 2003
Role of B800 in Carotenoid−Bacteriochlorophyll Energy and Electron Transfer in LH2 Complexes from the Purple Bacterium Rhodobacter sphaeroides journal June 2007
Ultrafast Formation of a Carotenoid Radical in LH2 Antenna Complexes of Purple Bacteria journal September 2004
Mechanism of the Carotenoid-to-Bacteriochlorophyll Energy Transfer via the S 1 State in the LH2 Complexes from Purple Bacteria journal April 2000
Efficient Energy Transfer from the Carotenoid S2 State in a Photosynthetic Light-Harvesting Complex journal February 2001
The Carotenoid S 1 State in LH2 Complexes from Purple Bacteria Rhodobacter s phaeroides and Rhodopseudomonas a cidophila :  S 1 Energies, Dynamics, and Carotenoid Radical Formation journal October 2002
Mechanism of Energy Transfer from Carotenoids to Bacteriochlorophyll:  Light-Harvesting by Carotenoids Having Different Extents of π-Electron Conjugation Incorporated into the B850 Antenna Complex from the Carotenoidless Bacterium Rhodobacter sphaeroides R-26.1 journal October 1998
A spectral characterisation of the light-harvesting pigment-protein complexes from Rhodopseudomonas acidophila journal March 1986
Effect of a conjugated carbonyl group on the photophysical properties of carotenoids journal January 2004
Identification of a mechanism of photoprotective energy dissipation in higher plants journal November 2007
A Mechanism of Energy Dissipation in Cyanobacteria journal March 2009
Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins journal February 2015
Self-Trapped Excitons in LH2 Antenna Complexes between 5 K and Ambient Temperature journal October 2003
Fluorescence and photobleaching dynamics of single light-harvesting complexes journal September 1997
Comparison of the fluorescence kinetics of detergent-solubilized and membrane-reconstituted LH2 complexes from Rps. acidophila and Rb. sphaeroides journal October 2007
The 2Ag− energies of all-trans-neurosporene and spheroidene as determined by fluorescence spectroscopy journal May 1998
Excited-state dynamics among the 1Bu+, 1Bu− and 2Ag− states of all-trans-neurosporene as revealed by near-infrared time-resolved absorption spectroscopy journal December 2000
A first detection of singlet to triplet conversion from the 11Bu− to the 13Ag state and triplet internal conversion from the 13Ag to the 13Bu state in carotenoids: dependence on the conjugation length journal July 2003
Effect of Isomer Geometry on the Steady-State Absorption Spectra and Femtosecond Time-Resolved Dynamics of Carotenoids journal November 2005
The dynamics of the S1 excited states of carotenoids journal May 1993
Fluorescence Spectroscopy of All- trans -anhydrorhodovibrin and Spirilloxanthin:  Detection of the 1B u - Fluorescence journal June 2001