Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif

Park, Eunyoung; Graziano, Brian R.; Zheng, Wei; Garabedian, Mikael; Goode, Bruce L.; Eck, Michael J.

doi:10.1016/j.str.2015.05.015

Title: Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif

Journal Article · Thu Aug 06 00:00:00 EDT 2015 · Structure

DOI:https://doi.org/10.1016/j.str.2015.05.015· OSTI ID:1209697

Park, Eunyoung; Graziano, Brian R.; Zheng, Wei; Garabedian, Mikael; Goode, Bruce L.; Eck, Michael J. ^[1]

DFCI

In budding yeast, the actin-binding protein Bud6 cooperates with formins Bni1 and Bnr1 to catalyze the assembly of actin filaments. The nucleation-enhancing activity of Bud6 requires both a “core” domain that binds to the formin and a “flank” domain that binds monomeric actin. Here, we describe the structure of the Bud6 flank domain in complex with actin. Two helices in Bud6^flank interact with actin; one binds in a groove at the barbed end of the actin monomer in a manner closely resembling the helix of WH2 domains, a motif found in many actin nucleation factors. The second helix rises along the face of actin. Mutational analysis verifies the importance of these Bud6-actin contacts for nucleation-enhancing activity. The Bud6 binding site on actin overlaps with that of the formin FH2 domain and is also incompatible with inter-subunit contacts in F-actin, suggesting that Bud6 interacts only transiently with actin monomers during filament nucleation.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH)

OSTI ID:: 1209697

Journal Information:: Structure, Vol. 23, Issue (8) ; 08, 2015

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 13 works

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