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Title: PNEUMATIC MICROVALVE FOR ELECTROKINETIC SAMPLE PRECONCENTRATION AND CAPILLARY ELECTROPHORESIS INJECTION

Here we show that a closed pneumatic microvalve on a PDMS chip can serve as a semipermeable membrane under an applied potential, enabling current to pass through while blocking the passage of charged analytes. Enrichment of both anionic and cationic species has been demonstrated, and concentration factors of ~70 have been achieved in just 8 s. Once analytes are concentrated, the valve is briefly opened and the sample is hydrodynamically injected onto an integrated microchip or capillary electrophoresis (CE) column. In contrast to existing preconcentration approaches, the membrane-based method described here enables both rapid analyte concentration as well as high resolution separations.
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
1194323
Report Number(s):
PNNL-SA-107701
48204; KP1704020
DOE Contract Number:
AC05-76RL01830
Resource Type:
Conference
Resource Relation:
Conference: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, October 26-30, 2014, San Antonio, Texas, 2560-2561
Publisher:
Royal Society of Chemistry, London, United Kingdom.
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
Preconcentration, Injection, Membrane, Microchip Electrophoresis; Environmental Molecular Sciences Laboratory