skip to main content

SciTech ConnectSciTech Connect

Title: Construction of an in vitro primary lung co-culture platform derived from New Zealand white rabbits

We report the construction of an in vitro three dimensional (3D) co-culture platform consisting of differentiated lung epithelial cells and monocytes from New Zealand white rabbits. Rabbit lung epithelial cells were successfully grown at air-liquid interface, produced mucus, and expressed both sialic acid alpha-2,3 and alpha-2,6. Blood-derived CD14+ monocytes were deposited above the epithelial layer resulting in the differentiation of a subset of monocytes into CD11c+ cells within the co-culture. These proof-of-concept findings provide a convenient means to comparatively study in vitro versus in vivo rabbit lung responses as they relate to inhalation or lung-challenge studies.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
1191805
Report Number(s):
PNNL-SA-104791
400904120
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: In Vitro Cellular & Developmental Biology – Animal, 51(5):433-440
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
rabbit; lung; primary epithelial