Construction of an in vitro primary lung co-culture platform derived from New Zealand white rabbits
Journal Article
·
· In Vitro Cellular & Developmental Biology – Animal, 51(5):433-440
We report the construction of an in vitro three dimensional (3D) co-culture platform consisting of differentiated lung epithelial cells and monocytes from New Zealand white rabbits. Rabbit lung epithelial cells were successfully grown at air-liquid interface, produced mucus, and expressed both sialic acid alpha-2,3 and alpha-2,6. Blood-derived CD14+ monocytes were deposited above the epithelial layer resulting in the differentiation of a subset of monocytes into CD11c+ cells within the co-culture. These proof-of-concept findings provide a convenient means to comparatively study in vitro versus in vivo rabbit lung responses as they relate to inhalation or lung-challenge studies.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1191805
- Report Number(s):
- PNNL-SA-104791; 400904120
- Journal Information:
- In Vitro Cellular & Developmental Biology – Animal, 51(5):433-440, Journal Name: In Vitro Cellular & Developmental Biology – Animal, 51(5):433-440
- Country of Publication:
- United States
- Language:
- English
Similar Records
An integrated experimental-computational approach for predicting virulence in New Zealand white rabbits and humans following inhalation exposure to Bacillus anthracis spores
Morphologic injury and lipid peroxidation in monolayer cultures of rabbit tracheal epithelium exposed in vitro to ozone
Effects of ozone on macrophage adhesion in vitro and epithelial and inflammatory responses in vivo: The role of cytokines
Journal Article
·
Mon Jul 01 00:00:00 EDT 2019
· PLoS ONE
·
OSTI ID:1191805
+7 more
Morphologic injury and lipid peroxidation in monolayer cultures of rabbit tracheal epithelium exposed in vitro to ozone
Journal Article
·
Wed Aug 01 00:00:00 EDT 1990
· Journal of Toxicology and Environmental Health; (USA)
·
OSTI ID:1191805
+1 more
Effects of ozone on macrophage adhesion in vitro and epithelial and inflammatory responses in vivo: The role of cytokines
Journal Article
·
Sat Feb 01 00:00:00 EST 1997
· Journal of Toxicology and Environmental Health
·
OSTI ID:1191805