An enhanced droplet-based liquid microjunction surface sampling system coupled with HPLC-ESI-MS/MS for spatially resolved analysis

Van Berkel, Gary J.; Weiskittel, Taylor M.; Kertesz, Vilmos

doi:10.1007/s00216-014-8287-5

Title: An enhanced droplet-based liquid microjunction surface sampling system coupled with HPLC-ESI-MS/MS for spatially resolved analysis

Journal Article · Fri Nov 07 00:00:00 EST 2014 · Analytical and Bioanalytical Chemistry

DOI:https://doi.org/10.1007/s00216-014-8287-5· OSTI ID:1185691

Van Berkel, Gary J. ^[1]; Weiskittel, Taylor M. ^[2]; Kertesz, Vilmos ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)

Droplet-based liquid microjunction surface sampling coupled with high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) for spatially resolved analysis provides the possibility of effective analysis of complex matrix samples and can provide a greater degree of chemical information from a single spot sample than is typically possible with a direct analysis of an extract. Described here is the setup and enhanced capabilities of a discrete droplet liquid microjunction surface sampling system employing a commercially available CTC PAL autosampler. The system enhancements include incorporation of a laser distance sensor enabling unattended analysis of samples and sample locations of dramatically disparate height as well as reliably dispensing just 0.5 μL of extraction solvent to make the liquid junction to the surface, wherein the extraction spot size was confined to an area about 0.7 mm in diameter; software modifications improving the spatial resolution of sampling spot selection from 1.0 to 0.1 mm; use of an open bed tray system to accommodate samples as large as whole-body rat thin tissue sections; and custom sample/solvent holders that shorten sampling time to approximately 1 min per sample. Lastly, the merit of these new features was demonstrated by spatially resolved sampling, HPLC separation, and mass spectral detection of pharmaceuticals and metabolites from whole-body rat thin tissue sections and razor blade (“crude”) cut mouse tissue.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: Work for Others (WFO); USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1185691

Journal Information:: Analytical and Bioanalytical Chemistry, Vol. 407, Issue 8; ISSN 1618-2642

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 27 works

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References (16)

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High-Throughput Mode Liquid Microjunction Surface Sampling Probe Van Berkel, Gary J.; Kertesz, Vilmos; King, Richard C. Analytical Chemistry, Vol. 81, Issue 16 https://doi.org/10.1021/ac901098d	journal	August 2009
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Tryptic Digestion Coupled with Ambient Desorption Electrospray Ionization and Liquid Extraction Surface Analysis Mass Spectrometry Enabling Identification of Skeletal Muscle Proteins in Mixtures and Distinguishing between Beef, Pork, Horse, Chicken, and Turkey Meat Montowska, Magdalena; Rao, Wei; Alexander, Morgan R. Analytical Chemistry, Vol. 86, Issue 9 https://doi.org/10.1021/ac5003432	journal	April 2014
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Comparison of Drug Distribution Images from Whole-Body Thin Tissue Sections Obtained Using Desorption Electrospray Ionization Tandem Mass Spectrometry and Autoradiography Kertesz, Vilmos; Van Berkel, Gary J.; Vavrek, Marissa Analytical Chemistry, Vol. 80, Issue 13 https://doi.org/10.1021/ac800546a	journal	July 2008
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Continuous-flow liquid microjunction surface sampling probe connected on-line with high-performance liquid chromatography/mass spectrometry for spatially resolved analysis of small molecules and proteins: LMJ-SPP on-line with HPLC/MS Van Berkel, Gary J.; Kertesz, Vilmos Rapid Communications in Mass Spectrometry, Vol. 27, Issue 12 https://doi.org/10.1002/rcm.6580	journal	May 2013

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Dereplicating and Spatial Mapping of Secondary Metabolites from Fungal Cultures in Situ Sica, Vincent P.; Raja, Huzefa A.; El-Elimat, Tamam Journal of Natural Products, Vol. 78, Issue 8 https://doi.org/10.1021/acs.jnatprod.5b00268	journal	July 2015
Substrate-Mediated Laser Ablation under Ambient Conditions for Spatially-Resolved Tissue Proteomics Fatou, Benoit; Wisztorski, Maxence; Focsa, Cristian Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep18135	journal	December 2015
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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
liquid microjunction
droplet-based liquid extraction
autosampler
spatial distribution
laser distance sensor

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