MICROFLUIDIC MODULES FOR ISOLATION OF RECOMBINANT CYTOKINE FROM BACTERIAL LYSATES

Conference · Wed Jan 01 00:00:00 EST 2014

OSTI ID:1185594

Millet, Larry J ^[1]; Retterer, Scott T ^[1]; Doktycz, Mitchel John ^[1]

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1. ORNL

The portability and personalization of health-care diagnostics and treatments benefits from advancements and applications of micro and nanotechnology. Modularization and miniaturization of standardized biochemical processes and tests facilitates the advancement and customization of analyte detection and diagnosis on-chip. The goal of our work here is to develop modular platforms for on-chip biochemical processing of synthesized biologics for a range of on-demand applications. Our report focuses on the initial development, characterization and application of microfluidic size exclusion/gel filtration and ion exchange protein concentration modules for cytokine isolation from spiked cell extracts.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
• Sponsoring Organization: Work for Others (WFO)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1185594
• Resource Relation: Conference: microTAS 2014, San Antonio, TX, USA, 20141026, 20141030
• Country of Publication: United States
• Language: English