Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype
Trichoderma reesei is the main industrial source of cellulases and hemicellulases required for the hydrolysis of biomass to simple sugars, which can then be used in the production of biofuels and biorefineries. The highly productive strains in use today were generated by classical mutagenesis. As byproducts of this procedure, mutants were generated that turned out to be unable to produce cellulases. In order to identify the mutations responsible for this inability, we sequenced the genome of one of these strains, QM9136, and compared it to that of its progenitor T. reesei QM6a.
- Vienna Univ. of Technology, Vienna (Austria)
- IFP Energies Nouvelles, Rueil-Malmaison (France)
- Sorbonne Univ., Paris (France)
- Joint Genome Inst., Walnut Creek, CA (United States). US Dept. of Energy.
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Publication Date:
- OSTI Identifier:
- Grant/Contract Number:
- Accepted Manuscript
- Journal Name:
- BMC Genomics
- Additional Journal Information:
- Journal Volume: 16; Journal Issue: 1; Journal ID: ISSN 1471-2164
- Research Org:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org:
- Country of Publication:
- United States
- 59 BASIC BIOLOGICAL SCIENCES single nucleotide polymorphism; SNP; indel; comparative genomics; classical mutant; XYR1; transcription; factor shuttling; cellulases; Trichoderma reesei; QM9136
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