Characterization of Hydrogen Metabolism in the Multicellular Green Alga Volvox carteri

Cornish, Adam J.; Green, Robin; Gärtner, Katrin; Mason, Saundra; Hegg, Eric L.

doi:10.1371/journal.pone.0125324

Title: Characterization of Hydrogen Metabolism in the Multicellular Green Alga Volvox carteri

Journal Article · Thu Apr 30 00:00:00 EDT 2015 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0125324· OSTI ID:1184789

Cornish, Adam J. ^[1]; Green, Robin ^[1]; Gärtner, Katrin ^[1]; Mason, Saundra ^[1]; Hegg, Eric L. ^[1]

Great Lakes Bioenergy Research Center, East Lansing, MI (United States); Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology

Hydrogen gas functions as a key component in the metabolism of a wide variety of microorganisms, often acting as either a fermentative end-product or an energy source. The number of organisms reported to utilize hydrogen continues to grow, contributing to and expanding our knowledge of biological hydrogen processes. Here we demonstrate that Volvox carteri f. nagariensis, a multicellular green alga with differentiated cells, evolves H₂ both when supplied with an abiotic electron donor and under physiological conditions. The genome of Volvox carteri contains two genes encoding putative [FeFe]-hydrogenases (HYDA1 and HYDA2), and the transcripts for these genes accumulate under anaerobic conditions. The HYDA1 and HYDA2 gene products were cloned, expressed, and purified, and both are functional [FeFe]-hydrogenases. Additionally, within the genome the HYDA1 and HYDA2 genes cluster with two putative genes which encode hydrogenase maturation proteins. This gene cluster resembles operon-like structures found within bacterial genomes and may provide further insight into evolutionary relationships between bacterial and algal [FeFe]-hydrogenase genes.

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Research Organization:: Great Lakes Bioenergy Research Center, East Lansing, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1184789

Journal Information:: PLoS ONE, Vol. 10, Issue 4; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 6 works

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