Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation

Li, Jie; Nakayasu, Ernesto S.; Overall, Christopher C.; Johnson, Rudd C.; Kidwai, Afshan S.; McDermott, Jason E.; Ansong, Charles; Heffron, Fred; Cambronne, Eric D.; Adkins, Joshua N.

doi:10.1021/pr5010423

Title: Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation

Journal Article · Mon Feb 16 00:00:00 EST 2015 · Journal of Proteome Research

DOI:https://doi.org/10.1021/pr5010423· OSTI ID:1182899

Li, Jie ^[1]; Nakayasu, Ernesto S. ^[2]; Overall, Christopher C. ^[2]; Johnson, Rudd C. ^[1]; Kidwai, Afshan S. ^[1]; McDermott, Jason E. ^[2]; Ansong, Charles ^[2]; Heffron, Fred ^[1]; Cambronne, Eric D. ^[1]; Adkins, Joshua N. ^[2]

Oregon Health & Science Univ., Portland, OR (United States). Dept. of Molecular Microbiology and Immunology
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division

The alternative sigma factor E (σ^E) is critical for response to extracytoplasmic stress in Salmonella. Extensive studies have been conducted on σ^E-regulated gene expression, particularly at the transcriptional level. Increasing evidence suggests however that σ^E may indirectly participate in post-transcriptional regulation. Here in this study, we conducted sample-matched global proteomic and transcriptomic analyses to determine the level of regulation mediated by σ^E in Salmonella. We analysed samples from wild type and isogenic rpoE mutant Salmonella cultivated in three different conditions; nutrient-rich and conditions that mimic early and late intracellular infection. We found that 30% of the observed proteome was regulated by σ^E combining all three conditions. In different growth conditions, σ^E affected the expression of a broad spectrum of Salmonella proteins required for miscellaneous functions. Those involved in transport and binding, protein synthesis, and stress response were particularly highlighted. By comparing transcriptomic and proteomic data, we identified genes post-transcriptionally regulated by σ^E and found that post-transcriptional regulation was responsible for a majority of changes observed in the σ^E-regulated proteome. Further, comparison of transcriptomic and proteomic data from hfq mutant of Salmonella demonstrated that σ^E–mediated post-transcriptional regulation was partially dependent on the RNA-binding protein Hfq.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH)

Grant/Contract Number:: AC05-76RLO01830; GM094623; GM103493

OSTI ID:: 1182899

Report Number(s):: PNNL-SA-105707; 42294; 47623; 48135; 47418; 400412000

Journal Information:: Journal of Proteome Research, Vol. 14, Issue 4; ISSN 1535-3893

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 11 works

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Caco-2 Invasion by Cronobacter sakazakii and Salmonella enterica Exposed to Drying and Heat Treatments in Dried State in Milk Powder Lang, Emilie; Guyot, Stéphane; Alvarez-Martin, Pablo Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.01893	journal	September 2017
A σE-Mediated Temperature Gauge Controls a Switch from LuxR-Mediated Virulence Gene Expression to Thermal Stress Adaptation in Vibrio alginolyticus Gu, Dan; Guo, Min; Yang, Minjun PLOS Pathogens, Vol. 12, Issue 6 https://doi.org/10.1371/journal.ppat.1005645	journal	June 2016
Identification of Novel Host Interactors of Effectors Secreted by Salmonella and Citrobacter Sontag, Ryan L.; Nakayasu, Ernesto S.; Brown, Roslyn N. mSystems, Vol. 1, Issue 4 https://doi.org/10.1128/msystems.00032-15	journal	July 2016
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Alternative Sigma Factor RpoX Is a Part of the RpoE Regulon and Plays Distinct Roles in Stress Responses, Motility, Biofilm Formation, and Hemolytic Activities in the Marine Pathogen Vibrio alginolyticus Gu, Dan; Zhang, Jun; Hao, Yuan Applied and Environmental Microbiology, Vol. 85, Issue 14 https://doi.org/10.1128/aem.00234-19	journal	May 2019

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59 BASIC BIOLOGICAL SCIENCES
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Title: Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation

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