DNA-mediated engineering of multicomponent enzyme crystals
Abstract
The ability to predictably control the coassembly of multiple nanoscale building blocks, especially those with disparate chemical and physical properties such as biomolecules and inorganic nanoparticles, has far-reaching implications in catalysis, sensing, and photonics, but a generalizable strategy for engineering specific contacts between these particles is an outstanding challenge. This is especially true in the case of proteins, where the types of possible interparticle interactions are numerous, diverse, and complex. In this paper, we explore the concept of trading protein–protein interactions for DNA–DNA interactions to direct the assembly of two nucleic-acid–functionalized proteins with distinct surface chemistries into six unique lattices composed of catalytically active proteins, or of a combination of proteins and DNA-modified gold nanoparticles. The programmable nature of DNA–DNA interactions used in this strategy allows us to control the lattice symmetries and unit cell constants, as well as the compositions and habit, of the resulting crystals. Finally, this study provides a potentially generalizable strategy for constructing a unique class of materials that take advantage of the diverse morphologies, surface chemistries, and functionalities of proteins for assembling functional crystalline materials.
- Authors:
-
- Northwestern Univ., Evanston, IL (United States). Dept. of Chemistry. International Inst. for Nanotechnology
- Northwestern Univ., Evanston, IL (United States). International Inst. for Nanotechnology. Dept. of Materials Science and Engineering
- Northwestern Univ., Evanston, IL (United States). Dept. of Chemistry. International Inst. for Nanotechnology. Dept. of Materials Science and Engineering
- Publication Date:
- Research Org.:
- Northwestern Univ., Evanston, IL (United States)
- Sponsoring Org.:
- USDOE; USDOD; US Air Force Office of Scientific Research (AFOSR)
- OSTI Identifier:
- 1182330
- Grant/Contract Number:
- AC02-06CH11357; N00014-15-1-0043; FA9550-11-1-0275
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Volume: 112; Journal Issue: 15; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; nanoscience; biomaterials; self-assembly; superlattice; DNA-programmable assembly
Citation Formats
Brodin, Jeffrey D., Auyeung, Evelyn, and Mirkin, Chad A. DNA-mediated engineering of multicomponent enzyme crystals. United States: N. p., 2015.
Web. doi:10.1073/pnas.1503533112.
Brodin, Jeffrey D., Auyeung, Evelyn, & Mirkin, Chad A. DNA-mediated engineering of multicomponent enzyme crystals. United States. https://doi.org/10.1073/pnas.1503533112
Brodin, Jeffrey D., Auyeung, Evelyn, and Mirkin, Chad A. 2015.
"DNA-mediated engineering of multicomponent enzyme crystals". United States. https://doi.org/10.1073/pnas.1503533112. https://www.osti.gov/servlets/purl/1182330.
@article{osti_1182330,
title = {DNA-mediated engineering of multicomponent enzyme crystals},
author = {Brodin, Jeffrey D. and Auyeung, Evelyn and Mirkin, Chad A.},
abstractNote = {The ability to predictably control the coassembly of multiple nanoscale building blocks, especially those with disparate chemical and physical properties such as biomolecules and inorganic nanoparticles, has far-reaching implications in catalysis, sensing, and photonics, but a generalizable strategy for engineering specific contacts between these particles is an outstanding challenge. This is especially true in the case of proteins, where the types of possible interparticle interactions are numerous, diverse, and complex. In this paper, we explore the concept of trading protein–protein interactions for DNA–DNA interactions to direct the assembly of two nucleic-acid–functionalized proteins with distinct surface chemistries into six unique lattices composed of catalytically active proteins, or of a combination of proteins and DNA-modified gold nanoparticles. The programmable nature of DNA–DNA interactions used in this strategy allows us to control the lattice symmetries and unit cell constants, as well as the compositions and habit, of the resulting crystals. Finally, this study provides a potentially generalizable strategy for constructing a unique class of materials that take advantage of the diverse morphologies, surface chemistries, and functionalities of proteins for assembling functional crystalline materials.},
doi = {10.1073/pnas.1503533112},
url = {https://www.osti.gov/biblio/1182330},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
issn = {0027-8424},
number = 15,
volume = 112,
place = {United States},
year = {Mon Mar 23 00:00:00 EDT 2015},
month = {Mon Mar 23 00:00:00 EDT 2015}
}
Web of Science
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