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This content will become publicly available on March 23, 2016

Title: DNA-mediated engineering of multicomponent enzyme crystals

The ability to predictably control the coassembly of multiple nanoscale building blocks, especially those with disparate chemical and physical properties such as biomolecules and inorganic nanoparticles, has far-reaching implications in catalysis, sensing, and photonics, but a generalizable strategy for engineering specific contacts between these particles is an outstanding challenge. This is especially true in the case of proteins, where the types of possible interparticle interactions are numerous, diverse, and complex. In this paper, we explore the concept of trading protein–protein interactions for DNA–DNA interactions to direct the assembly of two nucleic-acid–functionalized proteins with distinct surface chemistries into six unique lattices composed of catalytically active proteins, or of a combination of proteins and DNA-modified gold nanoparticles. The programmable nature of DNA–DNA interactions used in this strategy allows us to control the lattice symmetries and unit cell constants, as well as the compositions and habit, of the resulting crystals. Finally, this study provides a potentially generalizable strategy for constructing a unique class of materials that take advantage of the diverse morphologies, surface chemistries, and functionalities of proteins for assembling functional crystalline materials.
Authors:
 [1] ;  [2] ;  [3]
  1. Northwestern Univ., Evanston, IL (United States). Dept. of Chemistry. International Inst. for Nanotechnology
  2. Northwestern Univ., Evanston, IL (United States). International Inst. for Nanotechnology. Dept. of Materials Science and Engineering
  3. Northwestern Univ., Evanston, IL (United States). Dept. of Chemistry. International Inst. for Nanotechnology. Dept. of Materials Science and Engineering
Publication Date:
OSTI Identifier:
1182330
Grant/Contract Number:
AC02-06CH11357; N00014-15-1-0043; FA9550-11-1-0275
Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 112; Journal Issue: 15; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Research Org:
Northwestern Univ., Evanston, IL (United States)
Sponsoring Org:
USDOE; USDOD; US Air Force Office of Scientific Research (AFOSR)
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; nanoscience; biomaterials; self-assembly; superlattice; DNA-programmable assembly