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Title: Functional reconstitution of Drosophila melanogaster NMJ glutamate receptors

The Drosophila larval neuromuscular junction (NMJ), at which glutamate acts as the excitatory neurotransmitter, is a widely used model for genetic analysis of synapse function and development. Despite decades of study, the inability to reconstitute NMJ glutamate receptor function using heterologous expression systems has complicated the analysis of receptor function, such that it is difficult to resolve the molecular basis for compound phenotypes observed in mutant flies. In this paper, we find that Drosophila Neto functions as an essential component required for the function of NMJ glutamate receptors, permitting analysis of glutamate receptor responses in Xenopus oocytes. Finally, in combination with a crystallographic analysis of the GluRIIB ligand binding domain, we use this system to characterize the subunit dependence of assembly, channel block, and ligand selectivity for Drosophila NMJ glutamate receptors.
Authors:
 [1] ;  [2] ;  [2] ;  [1]
  1. National Inst. of Health, Bethesda, MD (United States). The Eunice Kennedy Shriver National Inst. of Child Health and Human Development. Program in Cellular Regulation and Metabolism
  2. National Inst. of Health, Bethesda, MD (United States). The Eunice Kennedy Shriver National Inst. of Child Health and Human Development. Lab. of Cellular and Molecular Neurophysiology
Publication Date:
OSTI Identifier:
1182323
Grant/Contract Number:
W-31-109-Eng-38
Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 112; Journal Issue: 19; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Research Org:
National Inst. of Health, Bethesda, MD (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Inst. of Health (NIH) (United States)
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Drosophila; NMJ; Neto; glutamate receptors; crystallography