Functional reconstitution of Drosophila melanogaster NMJ glutamate receptors

Han, Tae Hee; Dharkar, Poorva; Mayer, Mark L.; Serpe, Mihaela

doi:10.1073/pnas.1500458112

Title: Functional reconstitution of Drosophila melanogaster NMJ glutamate receptors

Journal Article · Mon Apr 27 00:00:00 EDT 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1500458112· OSTI ID:1182323

Han, Tae Hee ^[1]; Dharkar, Poorva ^[2]; Mayer, Mark L. ^[2]; Serpe, Mihaela ^[1]

National Inst. of Health, Bethesda, MD (United States). The Eunice Kennedy Shriver National Inst. of Child Health and Human Development. Program in Cellular Regulation and Metabolism
National Inst. of Health, Bethesda, MD (United States). The Eunice Kennedy Shriver National Inst. of Child Health and Human Development. Lab. of Cellular and Molecular Neurophysiology

The Drosophila larval neuromuscular junction (NMJ), at which glutamate acts as the excitatory neurotransmitter, is a widely used model for genetic analysis of synapse function and development. Despite decades of study, the inability to reconstitute NMJ glutamate receptor function using heterologous expression systems has complicated the analysis of receptor function, such that it is difficult to resolve the molecular basis for compound phenotypes observed in mutant flies. In this paper, we find that Drosophila Neto functions as an essential component required for the function of NMJ glutamate receptors, permitting analysis of glutamate receptor responses in Xenopus oocytes. Finally, in combination with a crystallographic analysis of the GluRIIB ligand binding domain, we use this system to characterize the subunit dependence of assembly, channel block, and ligand selectivity for Drosophila NMJ glutamate receptors.

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Research Organization:: National Inst. of Health, Bethesda, MD (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health (NIH) (United States)

Grant/Contract Number:: W-31-109-Eng-38

OSTI ID:: 1182323

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 19; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 33 works

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59 BASIC BIOLOGICAL SCIENCES
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Title: Functional reconstitution of Drosophila melanogaster NMJ glutamate receptors

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