Devices, systems, and methods for microscale isoelectric fractionation

Sommer, Gregory J; Hatch, Anson V; Wang, Ying-Chih; Singh, Anup K

Title: Devices, systems, and methods for microscale isoelectric fractionation

Patent · Tue Apr 14 00:00:00 EDT 2015

OSTI ID:1178015

Sommer, Gregory J; Hatch, Anson V; Wang, Ying-Chih; Singh, Anup K

Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.

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Research Organization:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: Sandia Corporation (Albuquerque, NM)

Patent Number(s):: 9,005,417

Application Number:: 12/243,817

OSTI ID:: 1178015

Resource Relation:: Patent File Date: 2008 Oct 01

Country of Publication:: United States

Language:: English

References (157)

Axial Bearing Muijderman, Everhardus A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/3376083 US Patent Document 3,376,083	patent	March 1966
Rotatable Finned Heat Transfer Device Bimshas, Jr., John; Hickey, Edward S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/3844341 US Patent Document 3,844,341	patent	October 1974
Separation of solid and liquid components of mixtures Hunter, William M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4125375 US Patent Document 4,125,375	patent	November 1978
Apparatus and method for measuring white blood cell and platelet concentrations in blood Wardlaw, Stephen C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4156570 US Patent Document 4,156,570	patent	May 1979
Compact miniature fan Muller, Rolf; Wrobel, Gunter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4164690 US Patent Document 4,164,690	patent	August 1979
Reversible drive circuit for brushless DC motor Uzuka, Mitsuo https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4282464 US Patent Document 4,282,464	patent	August 1981
Gas-lubricated bearings Beardmore, Geoffrey https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4380355 US Patent Document 4,380,355	patent	April 1983
Pre-column for preconcentrating substances to be chromatographed as well as device for a liquid chromatography system and coupling member for said device Ruijten, Henri M.; De Bree, Hans; Van Amsterdam, Peter H. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4554071 US Patent Document 4,554,071	patent	November 1985
Method and apparatus for measuring blood constituent counts Wardlaw, Stephen C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683579 US Patent Document 4,683,579	patent	July 1987
Dynamic heat sink Williams, Edward https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5000254 US Patent Document 5,000,254	patent	March 1991
Thermal control variable speed DC brushless fan Cheng, Ren-Hong https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5197858 US Patent Document 5,197,858	patent	March 1993
Method of separation employing magnetic particles and second medium Vorpahl, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5279936 US Patent Document 5,279,936	patent	January 1994
Cooling microfan arrangements and process Cronin, John; Previti-Kelly, Rosemary A.; Ryan, James G. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5296775 US Patent Document 5,296,775	patent	March 1994
Heat exchange apparatus and method for preparing the apparatus Ogushi, Tetsurou; Kaga, Kunihiko; Tanaka, Hideharu https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5297623 US Patent Document 5,297,623	patent	March 1994
Disk augmented heat transfer system Maling, Jr., George C.; Schmidt, Roger R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5335143 US Patent Document 5,335,143	patent	August 1994
Heat sink assembly for a central processing unit of a computer Wang, Michael https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5583746 US Patent Document 5,583,746	patent	December 1996
Fan motor Yamada, Tatsuo https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5616974 US Patent Document 5,616,974	patent	April 1997
Assay of blood or other biologic samples for target analytes Levine, Robert A.; Wardlaw, Stephen C.; Rodriguez, Rodolfo R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5635362 US Patent Document 5,635,362	patent	June 1997
Fan speed monitoring system for determining the speed of a PWM fan Brown, Alan E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5727928 US Patent Document 5,727,928	patent	March 1998
Transistor package structured to provide heat dissipation enabling use of silicon carbide transistors and other high power semiconductor devices Larimer, William R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5736787 US Patent Document 5,736,787	patent	April 1998
Forced air cooling apparatus for semiconductor chips Webster, Jr., Leo H.; Mok, Lawrence S.; Kamath, Vinod https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5794687 US Patent Document 5,794,687	patent	August 1998
Heat sink apparatus Amou, Mitsuru; Mehara, Koji https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5957659 US Patent Document 5,957,659	patent	September 1999
Apparatus for optimizing the rotational speed of cooling fans Giorgio, Paul J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5963887 US Patent Document 5,963,887	patent	October 1999
Cooling fan and cooling fan assembly Saito, Kohichi https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5979541 US Patent Document 5,979,541	patent	November 1999
Method and apparatus for cooling an electronic device Evans, Richard James; Gardell, David L.; Palagonia, Anthony M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6050326 US Patent Document 6,050,326	patent	April 2000
Data storage and/or retrieval methods and apparatuses and components thereof Fiske, Orlo James https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6078468 US Patent Document 6,078,468	patent	June 2000
Crystallizable compositions comprising a hepatitis C virus NS3 protease domain/NS4A complex Kim, Joseph L.; Morgenstern, Kurt; Lin, Chao https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6153579 US Patent Document 6,153,579	patent	November 2000
Heat transfer apparatus Batchelder, John Samuel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6175495 US Patent Document 6,175,495	patent	January 2001
Fan with magnetic blades Lopatinsky, Edward https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6194798 US Patent Document 6,194,798	patent	February 2001
Rotor drive motor with u-shaped stator cores Lopatinsky, Edward; Rosenfield, Saveliy T. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6249071 US Patent Document 6,249,071	patent	June 2001
Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system Mian, Alec; Kieffer-Higgins, Stephen; Corey, George https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6319469 US Patent Document 6,319,469	patent	November 2001
CPU fan assembly Davis, David R.; Flannery, Michael R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6356435 US Patent Document 6,356,435	patent	March 2002
Microfluidic systems Parce, John Wallace; Kopf-Sill, Anne R.; Bousse, Luc https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6379974 US Patent Document 6,379,974	patent	April 2002
Substrate structure of a liquid crystal display and a manufacturing method thereof Kim, Woong-Kwon https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6392720 US Patent Document 6,392,720	patent	May 2002
Composite heat dissipation fan Cheng, Jui-Hung https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6457955 US Patent Document 6,457,955	patent	October 2002
Circuit board fixing structure of heatsink fan Chen, Chien-Jung https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6525938 US Patent Document 6,525,938	patent	February 2003
Cooling module and related control circuits useful therefor incorporating a communication port for receiving digital command signals to control module Mays, II, Ford Chapman https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6545438 US Patent Document 6,545,438	patent	April 2003
Cooling apparatus for cooling electric element Watanabe, Mikio; Suzuki, Michihiro; Nakamura, Toshiyuki https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6619385 US Patent Document 6,619,385	patent	September 2003
Multilevel flow structures Hu, Xiaowen; Harwood, Mark; Boone, Travis https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6623860 US Patent Document 6,623,860	patent	September 2003
Method and device for separation of charged molecules by solution isoelectric focusing Speicher, David W.; Zuo, Xu https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6638408 US Patent Document 6,638,408	patent	October 2003
Cooler for electronic devices Lopatinsky, Edward; Fedoseyev, Lev; Fedosov, Yuriy Igorevich https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6659169 US Patent Document 6,659,169	patent	December 2003
Cooler for electronic devices Lopatinsky, Edward; Fedoseyev, Lev; Shaefer, Dan K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6664673 US Patent Document 6,664,673	patent	December 2003
Methods for forming small-volume electrical contacts and material manipulations with fluidic microchannels Jacobson, Stephen C.; Ramsey, J. Michael; Culbertson, Christopher T. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6685809 US Patent Document 6,685,809	patent	February 2004
Radiator fan Cheng, Jui-Hung https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6860323 US Patent Document 6,860,323	patent	March 2005
Very thin fan motor with attached heat sink Odagiri, Kinya; Oki, Kouji; Suzuki, Shingo https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6873069 US Patent Document 6,873,069	patent	March 2005
Active heat sink for high power microprocessors Sri-Jayantha, Sri M.; MoVicker, Gerard; Khanna, Vijayeshwar D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6876550 US Patent Document 6,876,550	patent	April 2005
Speed control circuit of brushless DC fan motor Xi, Junnan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6879120 US Patent Document 6,879,120	patent	April 2005
Hybrid cooling system and method for cooling electronic devices Al-Garni, Ahmed Z.; Hawwa, Muhammad A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6955215 US Patent Document 6,955,215	patent	October 2005
Class characterization of circulating cancer cells isolated from body fluids and methods of use Wang, Zheng; Ts'o, Paul O. P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6960449 US Patent Document 6,960,449	patent	November 2005
Venturi fan Herbert, Edward https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6966357 US Patent Document 6,966,357	patent	November 2005
Apparatus for cooling of electronic components Lopatinsky, Edward; Schaefer, Dan; Rosenfeld, Saveliy https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7021894 US Patent Document 7,021,894	patent	April 2006
Multi-parameter assays including analysis discs and methods relating thereto Gordon, John Francis https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7033747 US Patent Document 7,033,747	patent	April 2006
Apparatus for air cooling of an electronic device Holmes, Steven; Heirich, Douglas L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7035102 US Patent Document 7,035,102	patent	April 2006
Cooler for electronic devices Lopatinsky, Edward; Schaefer, Dan; Rosenfeld, Saveliy https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7044202 US Patent Document 7,044,202	patent	May 2006
Impeller driven active heat sink Roy, Sanjay https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7055581 US Patent Document 7,055,581	patent	June 2006
Integrated cooler for electronic devices Lopatinsky, Edward; Shaefer, Dan K.; Rosenfeld, Saveliy https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7071587 US Patent Document 7,071,587	patent	July 2006
Integrated liquid cooling system for electrical components Lee, Hsieh-Kun; Lai, Cheng-Tien; Zhou, Shi-Wen https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7100677 US Patent Document 7,100,677	patent	September 2006
Radial-flow heat-dissipating fan with increased inlet airflow Horng, Alex; Lu, Yu-Yuan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7134839 US Patent Document 7,134,839	patent	November 2006
Wave-fans and wave-fans with heat sinks Herbert, Edward https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7136285 US Patent Document 7,136,285	patent	November 2006
Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto Valencia, Ramoncito M.; Chen, YihFar; Hurt, Susan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7157049 US Patent Document 7,157,049	patent	January 2007
Integrated liquid cooling device with immersed electronic components Symons, Robert S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7165413 US Patent Document 7,165,413	patent	January 2007
Fan device for removing heat from heat sources Lee, Sen-Yung; Lin, Shueei-Muh; Hsieh, Hsin-Mao https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7165938 US Patent Document 7,165,938	patent	January 2007
CPU heat dissipating device structure Tsai, Ming-Kun https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7265975 US Patent Document 7,265,975	patent	September 2007
Heat-dissipating device Hsu, Wei-Chun; Chang, Shun-Chen; Huang, Wen-Shi https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7267526 US Patent Document 7,267,526	patent	September 2007
Cooling apparatus for heat generating devices Bahl, Manish; Mok, Lawrence S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7273091 US Patent Document 7,273,091	patent	September 2007
Heatsink assembly and method of manufacturing the same Larson, Ralph I. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7284596 US Patent Document 7,284,596	patent	October 2007
Heat-receiving apparatus and electronic equipment Hata, Yukihiko; Tomioka, Kentaro https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7301771 US Patent Document 7,301,771	patent	November 2007
Heat sink assembly Xia, Wan-Lin; Li, Tao; Zhong, Yong https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7304845 US Patent Document 7,304,845	patent	December 2007
Microfluidic particle-analysis systems Chou, Hou-Pu; Daridon, Antoine; Farrell, Kevin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7312085 US Patent Document 7,312,085	patent	December 2007
Dual impeller push-pull axial fan heat sink Foster, Sr., Jimmy G.; June, Michael S.; Makley, Albert Vincent https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7324339 US Patent Document 7,324,339	patent	January 2008
Heat dissipation device Xia, Wan-Lin; Li, Tao; Zhang, Jun https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7349212 US Patent Document 7,349,212	patent	March 2008
Fan motor Kaneko, Sachiko; Shishido, Yuji; Hashimoto, Toshio https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7381027 US Patent Document 7,381,027	patent	June 2008
Portable apparatus for separating sample and detecting target analytes Renzi, Ronald F.; Wally, Karl; Crocker, Robert W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7452507 US Patent Document 7,452,507	patent	November 2008
Miniature blower fan Horng, Alex; Yin, Tso-Kuo https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7455501 US Patent Document 7,455,501	patent	November 2008
Semiconductor chip heat transfer device Mok, Lawrence S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7458413 US Patent Document 7,458,413	patent	December 2008
Cooling apparatus Breier, Anton; Kamagata, Hidetoshi https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7481263 US Patent Document 7,481,263	patent	January 2009
Heat dissipation apparatus Hwang, Ching-Bai; Meng, Jin-Gong https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7520314 US Patent Document 7,520,314	patent	April 2009
Systems for integrated pump and reservoir Crocker, Michael T.; Carter, Daniel P.; Kozyra, Kazimierz L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7543457 US Patent Document 7,543,457	patent	June 2009
Pump structures integral to a fluid filled heat transfer apparatus Khanna, Vijayeshwar D.; McVicker, Gerard; Sri-Jayantha, Sri M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7667969 US Patent Document 7,667,969	patent	February 2010
Heat dissipating module Chang, Bor-Haw; Chou, Chu-Hsien https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7670102 US Patent Document 7,670,102	patent	March 2010
Miniature fan Horng, Alex; Yin, Tso-Kuo https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7695256 US Patent Document 7,695,256	patent	April 2010
Centrifugal force based microfluidic device, microfluidic system including the same, and method of determining home position of the microfluidic device Lee, Jeong-gun; Lee, Beom-seok; Park, Jong-myeon https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7758810 US Patent Document 7,758,810	patent	July 2010
Non-contacting thermal rotary joint Zimmerman, Kurt A.; Meehan, Robert Francis; Rust, John B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7836939 US Patent Document 7,836,939	patent	November 2010
Heat transfer device in a rotating structure Khanna, Vijayeshwar D.; McVicker, Gerard; Sri-Jayantha, Sri M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7896611 US Patent Document 7,896,611	patent	March 2011
Moving carbon nanotube heat sink Hawwa, Muhammad A.; Al-Dini, Salem A.; Ben-Mansour, Rached https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7900690 US Patent Document 7,900,690	patent	March 2011
Centrifugal pump device for a heat-dissipating system Huang, Kau-Chung https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7905712 US Patent Document 7,905,712	patent	March 2011
Heat sink for a circuit device Refai-Ahmed, Gamal; Touzelbaev, Maxat https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7911791 US Patent Document 7,911,791	patent	March 2011
Apparatus for precise transfer and manipulation of fluids by centrifugal and or capillary forces Pugia, Michael J.; Blankenstein, Gert; Peters, Ralf-Peter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8337775 US Patent Document 8,337,775	patent	December 2012
Devices and Method For Using Centripetal Acceleration To Drive Fluid Movement In A Microfluidics System With On-Board Informatics Mian, Alec; Kieffer-Higgins, Stephen G.; Corey, George D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20010055812 US Patent Document 08/761063; 20010055812	patent-application	December 2001
Composite heat dissipation fan Cheng, Jui-Hung https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020090307 US Patent Application 09/756661; 20020090307	patent-application	July 2002
Class characterization of circulating cancer cells isolated from body fluids and methods of use Wang, Zheng-Pin; TS'O, Paul O.P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020098535 US Patent Document 09/501179; 20020098535	patent-application	July 2002
Multichannel control in microfluidics Sassi, Alexander; Paulus, Aran https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020153251 US Patent Application 10/121378; 20020153251	patent-application	October 2002
Analytical methods and compositions Rao, Galla Chandra; Terstapper, Leon Wmm https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020164659 US Patent Application 10/017437; 20020164659	patent-application	November 2002
Plastic microfluidics enabling two-dimensional protein separations in proteome analysis Lee, Cheng Sheng; DeVoe, Don https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020170825 US Patent Application 10/135386; 20020170825	patent-application	November 2002
Microfluidic devices and methods Jedrzejewski, Paul; Nock, Steffen; Wagner, Peter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030013203 US Patent Application 10/208381; 20030013203	patent-application	January 2003
Method for separating cells Woodside, Steven M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030124719 US Patent Application 10/260560; 2003012471	patent-application	July 2003
Diffusion-based system and method for detecting and monitoring activity of biologic and chemical species Hefti, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030203504 US Patent Application 10/064392; 20030203504	patent-application	October 2003
Method of electrophoresis Bjellqvist, Bengt; Olsson, Ingmar; Palmgren, Ronnie https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030221963 US Patent Application 10/230526; 20030221963	patent-application	December 2003
Heat-dissipating device with dissipating fins drivable to move within and ambient fluid Jean, Amigo https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040035556 US Patent Application 10/614241; 20040035556	patent-application	February 2004
Microfluidic particle-analysis systems Chou, Hou-Pu; Daridon, Antoine; Farrell, Kevin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040072278 US Patent Application 10/405953; 20040072278	patent-application	April 2004
System and Method for Information Handling System Heat Sink Retention Kannmacher, Tracey; Jensen, Ralph W.; Boggs, Joshua M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040109291 US Patent Application 10/314074; 20040109291	patent-application	June 2004
Active heat sink for high power microprocessors Sri-Jayantha, Sri M.; MoVicker, Gerard; Khanna, Vijayeshwar Das https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040114327 US Patent Application 10/318700; 20040114327	patent-application	June 2004
MEMS based motor Takada, Akio; Yeo, Ching Biing; Cheng, Cheow Tong https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040119354 US Patent Application 10/730472; 20040119354	patent-application	June 2004
Apparatus for cooling of electronic components Lopatinsky, Edward; Schaefer, Dan; Rosenfeld, Saveliy https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050002163 US Patent Application 10/355864; 20050002163	patent-application	January 2005
Method and device for separation of charged molecules by solution isoelectric focusing Speicher, David W.; Zuo, Xun https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050087445 US Patent Application 10/996363; 20050087445	patent-application	April 2005
Heat dissipation module for a CPU Huang, Ming Tang https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050195573 US Patent Application 10/791883; 20050195573	patent-application	September 2005
Heatsink assembly and method of manufacturing the same Larson, Ralph I. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050274490 US Patent Application 10/722754; 20050274490	patent-application	December 2005
Integrated liquid cooling device with immersed electronic components Symons, Robert S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060007656 US Patent Application 11/056911; 20060007656	patent-application	January 2006
Integrated cooler for electronic devices Lopatinsky, Edward; Fedoseyev, Lev https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060021735 US Patent Application 11/185378; 20060021735	patent-application	February 2006
Integrated planar microfluidic bioanalytical systems Hawkins, Aaron R.; Peeni, Bridget; Barber, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060171654 US Patent Application 11/336646; 20060171654	patent-application	August 2006
Method and apparatus for gel electrophoretic immunoassay Herr, Amy Elizabeth; Singh, Anup K.; Throckmorton, Daniel J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060191792 US Patent Application 11/137745; 20060191792	patent-application	August 2006
Systems for integrated pump and reservoir Crocker, Michael T.; Carter, Daniel P.; Kozyra, Kazimierz L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070000268 US Patent Application 11/170427; 20070000268	patent-application	January 2007
Heat sink fastening system Hornung, Craig Warren https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070041158 US Patent Application 11/247018; 20070041158	patent-application	February 2007
Process and Apparatus for Heat Transfer Pelcz, Antal; Illes, Tamas; Horvath, Zoltan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070231419 US Patent Application 11/632276; 20070231419	patent-application	October 2007
Centrifugal pump device for a heat-dissipating system Huang, Kau-Chung https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080069706 US Patent Application 11/898680; 20080069706	patent-application	March 2008
Method Of Detecting Analyte-Molecule Interactions Tolley, Luke; McCarroll, Matt https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080149484 US Patent Application 11/923079; 20080149484	patent-application	June 2008
Cooling Device Walsh, Edmond; Grimes, Ronan; Punch, Jeff https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090145584 US Patent Application 12/084891; 20090145584	patent-application	June 2009
Heat Pipe Lai, Cheng-Tien; Zhou, Zhi-Yong; Ding, Qiao-Li https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090166004 US Patent Application 11/967058; 20090166004	patent-application	July 2009
Density-based cell detection system Kirakossian, Hrair https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100068754 US Patent Application 12/380877; 20100068754	patent	March 2010
Heat Exchanger Device and Method for Heat Removal or Transfer Koplow, Jeffrey P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100177480 US Patent Application 12/732662; 20100177480	patent-application	July 2010
Heat Sink for a Circuit Device Refai-Ahmed, Gamal; Touzelbaev, Maxat https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100328887 US Patent Application 12/490805; 20100328887	patent-application	December 2010
Heat Exchanger Device and Method for Heat Removal or Transfer Koplow, Jeffrey P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20110103011 US Patent Application 12/856440; 20110103011	patent-application	May 2011
Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886 https://doi.org/10.1021/ac050781s	journal	December 2005
Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device Das, Champak; Fan, Z. Hugh ELECTROPHORESIS, Vol. 27, Issue 18, p. 3619-3626 https://doi.org/10.1002/elps.200600013	journal	September 2006
Preparative-scale isoelectric trapping separations using a modified Gradiflow unit Ogle, David; Ho, Agnes; Gibson, Toby Journal of Chromatography A, Vol. 979, Issue 1-2, p. 155-161 https://doi.org/10.1016/S0021-9673(02)01601-1	journal	December 2002
A rapid direct fluorescent assay for cell-free DNA quantification in biological fluids Goldshtein, H.; Hausmann, M. J; Douvdevani, A. Annals of Clinical Biochemistry, Vol. 46, Issue 6, p. 488-494 https://doi.org/10.1258/acb.2009.009002	journal	September 2009
The current state of two-dimensional electrophoresis with immobilized pH gradients Görg, Angelika; Obermaier, Christian; Boguth, Günther Electrophoresis, Vol. 21, Issue 6, p. 1037-1053 https://doi.org/10.1002/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V	journal	April 2000
Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: Wide pH gradients up to pH 12, longer separation distances and simplified procedures Görg, Angelika; Obermaier, Christian; Boguth, Günther Electrophoresis, Vol. 20, Issue 4-5, p. 712-717 https://doi.org/10.1002/(SICI)1522-2683(19990101)20:4/5<712::AID-ELPS712>3.0.CO;2-Y	journal	January 1999
Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography Gusev, Isabelle; Huang, Xian; Horváth, Csaba Journal of Chromatography A, Vol. 855, Issue 1, p. 273-290 https://doi.org/10.1016/S0021-9673(99)00697-4	journal	September 1999
Parallel processing in the isoelectric focusing chip Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel ELECTROPHORESIS, Vol. 24, Issue 21, p. 3735-3744 https://doi.org/10.1002/elps.200305663	journal	November 2003
Parallel isoelectric focusing chip Zilberstein, Gleb; Korol, Leonid; Bukshpan, Shmuel PROTEOMICS, Vol. 4, Issue 9, p. 2533-2540 https://doi.org/10.1002/pmic.200300794	journal	September 2004
Parallel isoelectric focusing II Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel ELECTROPHORESIS, Vol. 25, Issue 21-22, p. 3643-3651 https://doi.org/10.1002/elps.200406117	journal	November 2004
Detecting Single Stranded DNA with a Solid State Nanopore Fologea, Daniel; Gershow, Marc; Ledden, Bradley Nano Letters, Vol. 5, Issue 10, p. 1905-1909 https://doi.org/10.1021/nl051199m	journal	October 2005
On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A. Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187 https://doi.org/10.1021/ac026239a	journal	March 2003
Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry Holmes, David; Pettigrew, David; Reccius, Christian H. Lab on a Chip, Vol. 9, Issue 20, p. 2881-2889 https://doi.org/10.1039/b910053a	journal	January 2009
Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient Huang, Tiemin; Pawliszyn, Janusz ELECTROPHORESIS, Vol. 23, Issue 20, p. 3504-3510 https://doi.org/10.1002/1522-2683(200210)23:20<3504::AID-ELPS3504>3.0.CO;2-X	journal	October 2002
A fully automated immunoassay from whole blood on a disc Lee, Beom Seok; Lee, Jung-Nam; Park, Jong-Myeon Lab on a Chip, Vol. 9, Issue 11, p. 1548-1555 https://doi.org/10.1039/b820321k	journal	January 2009
Bead-based microfluidic immunoassays: The next generation Lim, C. T.; Zhang, Y. Biosensors and Bioelectronics, Vol. 22, Issue 7, p. 1197-1204 https://doi.org/10.1016/j.bios.2006.06.005	journal	February 2007
Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing Lo, Catherine T.; Throckmorton, Daniel J.; Singh, Anup K. Lab on a Chip, Vol. 8, Issue 8, p. 1273-1279 https://doi.org/10.1039/b804485f	journal	January 2008
Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis Long, Zhicheng; Liu, Dayu; Ye, Nannan ELECTROPHORESIS, Vol. 27, Issue 24, p. 4927-4934 https://doi.org/10.1002/elps.200600252	journal	December 2006
Lab On A CD Madou, Marc; Zoval, Jim; Jia, Guangyao Annual Review of Biomedical Engineering, Vol. 8, Issue 1, p. 601-628 https://doi.org/10.1146/annurev.bioeng.8.061505.095758	journal	August 2006
Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry Maes, Melissa L.; Davidson, Lisa B.; McDonagh, Paul F. Journal of Immunological Methods, Vol. 319, Issue 1-2, p. 79-86 https://doi.org/10.1016/j.jim.2006.10.016	journal	January 2007
Functional integration of DNA purification and concentration into a real time micro-PCR chip Min, Junhong; Kim, Joon-Ho; Lee, Youngsun Lab on a Chip, Vol. 11, Issue 2, p. 259-265 https://doi.org/10.1039/C0LC00320D	journal	January 2011
Light scattering immunoassay Price, Christopher; Newman, David Principles and Practice of Immunoassay https://doi.org/10.1007/978-1-349-11234-0_16	book	January 1991
Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients Rhodes, Andrew; Wort, Stephen J.; Thomas, Helen Critical Care, Vol. 10, Issue 2, Article No. R60 https://doi.org/10.1186/cc4894	journal	April 2006
A B Cell-Based Sensor for Rapid Identification of Pathogens Rider, Todd H.; Petrovick, Martha S.; Nargi, Frances E. Science, Vol. 301, Issue 5630, p. 213-215 https://doi.org/10.1126/science.1084920	journal	July 2003
Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms Riegger, L.; Grumann, M.; Nann, T. Sensors and Actuators A: Physical, Vol. 126, Issue 2, p. 455-462 https://doi.org/10.1016/j.sna.2005.11.006	journal	February 2006
The Alpher, Bethe and Gamow of IEF, the alpha-Centaury of electrokinetic methodologies. Part II: Immobilized pH gradients Righetti, Pier Giorgio ELECTROPHORESIS, Vol. 28, Issue 4, p. 545-555 https://doi.org/10.1002/elps.200600256	journal	February 2007
The Alpher, Bethe, Gamow of isoelectric focusing, the alpha-Centaury of electrokinetic methodologies. Part I Righetti, Pier Giorgio ELECTROPHORESIS, Vol. 27, Issue 5-6, p. 923-938 https://doi.org/10.1002/elps.200500525	journal	March 2006
Design Optimization of Spirally Grooved Thrust Air Bearings for Polygon Mirror Laser Scanners Satomi, Tadaatsu; Lin, Gang JSME international journal. Ser. C, Dynamics, control, robotics, design and manufacturing, Vol. 36, Issue 3 https://doi.org/10.1299/jsmec1993.36.393	journal	January 1993
Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation Schaff, U. Y.; Sommer, G. J. Clinical Chemistry, Vol. 57, Issue 5, p. 753-761 https://doi.org/10.1373/clinchem.2011.162206	journal	March 2011
Miniaturized capillary isoelectric focusing in plastic microfluidic devices Tan, Woei; Fan, Z. Hugh; Qiu, Charmaine X. ELECTROPHORESIS, Vol. 23, Issue 20, p. 3638-3645 https://doi.org/10.1002/1522-2683(200210)23:20<3638::AID-ELPS3638>3.0.CO;2-Y	journal	October 2002
Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer Lim, Peniel; North, Robert; Vigh, Gyula ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859 https://doi.org/10.1002/elps.200600846	journal	June 2007
On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V. Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333 https://doi.org/10.1021/ac702523g	journal	May 2008
A Method for Global Analysis of Complex Proteomes Using Sample Prefractionation by Solution Isoelectrofocusing Prior to Two-Dimensional Electrophoresis Zuo, Xun; Speicher, David W. Analytical Biochemistry, Vol. 284, Issue 2 https://doi.org/10.1006/abio.2000.4714	journal	September 2000
A new biodosimetric method: branched DNA-based quantitative detection of B1 DNA in mouse plasma Zhang, L.; Zhang, M.; Yang, S. The British Journal of Radiology, Vol. 83, Issue 992, p. 694-701 https://doi.org/10.1259/bjr/49886569	journal	August 2010
Circulating DNA: a new diagnostic gold mine? Ziegler, Annemarie; Zangemeister-Wittke, Uwe; Stahel, Rolf A. Cancer Treatment Reviews, Vol. 28, Issue 5, p. 255-271 https://doi.org/10.1016/S0305-7372(02)00077-4	journal	October 2002

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