In vivo and in vitro olefin cyclopropanation catalyzed by heme enzymes

Coelho, Pedro S; Brustad, Eric M; Arnold, Frances H; Wang, Zhan; Lewis, Jared C

Title: In vivo and in vitro olefin cyclopropanation catalyzed by heme enzymes

Patent · Tue Mar 31 00:00:00 EDT 2015

OSTI ID:1177111

Coelho, Pedro S; Brustad, Eric M; Arnold, Frances H; Wang, Zhan; Lewis, Jared C

The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.

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Research Organization:: California Institute of Technology (CalTech), Pasadena, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-06ER15762

Assignee:: California Institute of Technology (Pasadena, CA)

Patent Number(s):: 8,993,262

Application Number:: 14/185,861

OSTI ID:: 1177111

Resource Relation:: Patent File Date: 2014 Feb 20

Country of Publication:: United States

Language:: English

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