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Title: Chimeric enzymes with improved cellulase activities

Patent ·
OSTI ID:1177097

Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-08GO28308
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
8,993,276
Application Number:
13/940,936
OSTI ID:
1177097
Resource Relation:
Patent File Date: 2013 Jul 12
Country of Publication:
United States
Language:
English

References (11)

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Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different journal April 2001
Cloning of Thermostable Cellulase Genes of Clostridium thermocellum and Their Secretive Expression in Bacillus subtilis journal November 2011
Multidomain and Multifunctional Glycosyl Hydrolases from the Extreme Thermophile Caldicellulosiruptor Isolate Tok7B.1 journal May 2000
Protein Disorder: Conformational Distribution of the Flexible Linker in a Chimeric Double Cellulase journal April 2005
Construction and characterization of chimeric cellulases with enhanced catalytic activity towards insoluble cellulosic substrates journal May 2012
Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725 journal January 2011
Optimizing the stability of single-chain proteins by linker length and composition mutagenesis journal May 1998
Cellulases and biofuels journal June 2009