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Title: Biocatalysts and methods for conversion of hemicellulose hydrolysates to biobased products

Patent ·
OSTI ID:1177095

The invention relates to processes and biocatalysts for producing ethanol and other useful products from biomass and/or other materials. Initial processing of lignocellulosic biomass frequently yields methylglucuronoxylose (MeGAX) and related products which are resistant to further processing by common biocatalysts. Strains of Enterobacter asburiae are shown to be useful in bioprocessing of MeGAX and other materials into useful bioproducts such as ethanol, acetate, lactate, and many others. Genetic engineering may be used to enhance production of desired bioproducts.

Research Organization:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC36-00GO10594
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Patent Number(s):
8,993,287
Application Number:
13/122,985
OSTI ID:
1177095
Resource Relation:
Patent File Date: 2010 May 27
Country of Publication:
United States
Language:
English

References (12)

Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes patent March 1991
Production of chemicals from lignocellulose, biomass or sugars patent August 2006
Materials and methods for efficient lactic acid production patent December 2009
Materials and methods for the efficient production of xylitol patent-application March 2007
Materials and Methods for the Efficient Production of Acetate and Other Products patent-application June 2009
Bacteria engineered for fuel ethanol production: current status journal December 2003
Genetic Engineering of Enterobacter asburiae Strain JDR-1 for Efficient Production of Ethanol from Hemicellulose Hydrolysates journal July 2009
Complete Fermentation of Xylose and Methylglucuronoxylose Derived from Methylglucuronoxylan by Enterobacter asburiae Strain JDR-1 journal November 2008
Purification and properties of an endo-1,4-xylanase excreted by a hydrolytic thermophilic anaerobe, Clostridium thermolacticum. A proposal for its action mechanism on larchwood 4-O-methylglucuronoxylan journal February 1990
Genetic engineering of Enterobacter asburiae strain JDR-1 for efficient d(−) lactic acid production from hemicellulose hydrolysate journal June 2009
Paenibacillus sp. Strain JDR-2 and XynA1: a Novel System for Methylglucuronoxylan Utilization journal February 2006
Gene Integration and Expression and Extracellular Secretion of Erwinia chrysanthemi Endoglucanase CelY (celY) and CelZ (celZ) in Ethanologenic Klebsiella oxytoca P2 journal January 2001

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