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Title: Double stranded nucleic acid biochips

This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.
Authors:
;
Publication Date:
OSTI Identifier:
1175749
Report Number(s):
7,049,073
10/283,670
DOE Contract Number:
W-31-109-ENG-38
Resource Type:
Patent
Research Org:
The University Of Chicago, Chicago, IL (United States) ; Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES