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Title: PCR amplification on microarrays of gel immobilized oligonucleotides

The invention relates two general methods for performing PCR amplification, combined with the detection and analysis of the PCR products on a microchip. In the first method, the amplification occurs both outside and within a plurality of gel pads on a microchip, with at least one oligonucleotide primer immobilized in a gel pad. In the second method, PCR amplification also takes place within gel pads on a microchip, but the pads are surrounded by a hydrophobic liquid such as that which separates the individual gel pads into environments which resemble micro-miniaturized test tubes.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
1174559
Report Number(s):
6,642,000
09/559,306
DOE Contract Number:
W-31-1-0-ENG-39
Resource Type:
Patent
Research Org:
University Of Chicago, Chicago, IL (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 42 ENGINEERING