Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor

Burg, John S.; Ingram, Jessica R.; Venkatakrishnan, A. J.; Jude, Kevin M.; Dukkipati, Abhiram; Feinberg, Evan N.; Angelini, Alessandro; Waghray, Deepa; Dror, Ron O.; Ploegh, Hidde L.; Garcia, K. Christopher

doi:10.1126/science.aaa5026

Title: Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor

Journal Article · Thu Mar 05 00:00:00 EST 2015 · Science

DOI:https://doi.org/10.1126/science.aaa5026· OSTI ID:1172427

Burg, John S.; Ingram, Jessica R.; Venkatakrishnan, A. J.; Jude, Kevin M.; Dukkipati, Abhiram; Feinberg, Evan N.; Angelini, Alessandro; Waghray, Deepa; Dror, Ron O.; Ploegh, Hidde L.; Garcia, K. Christopher ^[1]

Stanford

Chemokines are small proteins that function as immune modulators through activation of chemokine G protein-coupled receptors (GPCRs). Several viruses also encode chemokines and chemokine receptors to subvert the host immune response. How protein ligands activate GPCRs remains unknown. We report the crystal structure at 2.9 angstrom resolution of the human cytomegalovirus GPCR US28 in complex with the chemokine domain of human CX3CL1 (fractalkine). The globular body of CX3CL1 is perched on top of the US28 extracellular vestibule, whereas its amino terminus projects into the central core of US28. The transmembrane helices of US28 adopt an active-state-like conformation. Atomic-level simulations suggest that the agonist-independent activity of US28 may be due to an amino acid network evolved in the viral GPCR to destabilize the receptor’s inactive state.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: NIHOTHERHHMI

OSTI ID:: 1172427

Journal Information:: Science, Vol. 347, Issue 03, 2015

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 214 works

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