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Title: Antibacterial Drug Leads: DNA and Enzyme Multitargeting

Here, we report the results of an investigation of the activity of a series of amidine and bisamidine compounds against Staphylococcus aureus and Escherichia coli. The most active compounds bound to an AT-rich DNA dodecamer (CGCGAATTCGCG)2 and using DSC were found to increase the melting transition by up to 24 °C. Several compounds also inhibited undecaprenyl diphosphate synthase (UPPS) with IC50 values of 100–500 nM, and we found good correlations (R2 = 0.89, S. aureus; R2 = 0.79, E. coli) between experimental and predicted cell growth inhibition by using DNA ΔTm and UPPS IC50 experimental results together with one computed descriptor. Finally, we also solved the structures of three bisamidines binding to DNA as well as three UPPS structures. Overall, the results are of general interest in the context of the development of resistance-resistant antibiotics that involve multitargeting.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [2] ;  [3] ;  [1] ;  [2] ;  [1]
  1. Univ. of Illinois, Urbana-Champaign, IL (United States). Dept. of Chemistry
  2. Chinese Academy of Sciences (CAS), Tianjin (China). Inst. of Industrial Biotechnology, Industrial Enzymes National Engineering Lab.
  3. Academia Sinica, Taipei (Taiwan). Inst. of Biological Chemistry
Publication Date:
OSTI Identifier:
1172407
Grant/Contract Number:
AC02-06CH11357; 085P1000817; 2011CB710800; 2011CBA00805; 31200053; 31300615
Type:
Accepted Manuscript
Journal Name:
Journal of Medicinal Chemistry
Additional Journal Information:
Journal Volume: 58; Journal Issue: 3; Journal ID: ISSN 0022-2623
Publisher:
American Chemical Society (ACS)
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH); National Basic Research Program of China; National Natural Science Foundation of China (NNSFC)
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; Drug discovery; Protein structure; DNA minor groove binder; Undecaprenyl diphosphate synthase*