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Title: ON-DEMAND SERIAL DILUTION USING QUANTIZED NANO/PICOLITER-SCALE DROPLETS

This paper describes a fully automated droplet-based microfluidic device for on-demand serial dilution that is capable of achieving a dilution ratio of >6000 (concentration ranges from 1 mM to 160nM) over 35 nanoliter-scale droplets. This serial diluter can be applied to high throughput and label-free kinetic assays by integrating with our previously developed on-demand droplet-based microfluidic with mass spectrometry detection.
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
1171898
Report Number(s):
PNNL-SA-107700
47618; KP1704020
DOE Contract Number:
AC05-76RL01830
Resource Type:
Conference
Resource Relation:
Conference: Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), October 26-30, 2014, San Antonio, Texas, 1247-1249
Publisher:
Chemical and Biological Microsystems Society, San Diego, CA, United States(US).
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
Droplet microfluidics, Serial dilution, Concentration gradient, Mass spectrometry; Environmental Molecular Sciences Laboratory