skip to main content

SciTech ConnectSciTech Connect

Title: A Method to determine lysine acetylation stoichiometries

A major bottleneck to fully understanding the functional aspects of lysine acetylation is the lack of stoichiometry information. Here we describe a mass spectrometry method using a combination of isotope labeling and detection of a diagnostic fragment ion to determine the stoichiometry of lysine acetylation on proteins globally. Using this technique, we determined the modification occupancy on hundreds of acetylated peptides from cell lysates and cross-validated the measurements via immunoblotting.
Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
OSTI Identifier:
1171295
Report Number(s):
PNNL-SA-96571
42294; 400412000
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Proteomics, 2014:Article No. 730725
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory