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Title: Crystal structure of a fully glycosylated HIV-1 gp120 core reveals a stabilizing role for the glycan at Asn262

The crystal structure of a fully glycosylated HIV-1 gp120 core in complex with CD4 receptor and Fab 17b at 4.5-Å resolution reveals 9 of the 15 N-linked glycans of core gp120 to be partially ordered. The glycan at position Asn262 had the most extensive and well-ordered electron density, and a GlcNAc2Man7 was modeled. Lastly, the GlcNAc stem of this glycan is largely buried in a cleft in gp120, suggesting a role in gp120 folding and stability. Its arms interact with the stems of neighboring glycans from the oligomannose patch, which is a major target for broadly neutralizing antibodies.
Authors:
 [1] ;  [2] ;  [3]
  1. National Inst. of Health (NIH), Bethesda, MD (United States). Vaccine Research Center; Scripps Research Inst., La Jolla, CA (United States). Dept. of Integrative Structural and Computational Biology; Scripps Research Inst., La Jolla, CA (United States). International AIDS Vaccine Initiative Neutralizing Antibody Center; Scripps Research Inst., La Jolla, CA (United States). Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery
  2. Scripps Research Inst., La Jolla, CA (United States). Dept. of Integrative Structural and Computational Biology; Scripps Research Inst., La Jolla, CA (United States). International AIDS Vaccine Initiative Neutralizing Antibody Center; Scripps Research Inst., La Jolla, CA (United States). Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery; Scripps Research Inst., La Jolla, CA (United States). Skaggs Inst. for Chemical Biology
  3. National Inst. of Health (NIH), Bethesda, MD (United States). Vaccine Research Center
Publication Date:
OSTI Identifier:
1170033
Grant/Contract Number:
W-31-109-Eng-38; RO1 AI084817; GM U54 GM94586; UM1 AI100663
Type:
Accepted Manuscript
Journal Name:
Proteins
Additional Journal Information:
Journal Volume: 83; Journal Issue: 3; Journal ID: ISSN 0887-3585
Publisher:
Wiley
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
ENGLISH
Subject:
60 APPLIED LIFE SCIENCES; HIV-1 gp120; glycan shield; role of N262