Controlled Activation of Protein Rotational Dynamics Using Smart Hydrogel Tethering

Beech, Brenda M.; Xiong, Yijia; Boschek, Curt B.; Baird, Cheryl L.; Bigelow, Diana J.; Mcateer, Kathleen; Squier, Thomas C.

doi:10.1021/ja506717v

Title: Controlled Activation of Protein Rotational Dynamics Using Smart Hydrogel Tethering

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Abstract

Stimulus-responsive hydrogel materials that stabilize and control protein dynamics have the potential to enable a range of applications to take advantage of the inherent specificity and catalytic efficiencies of proteins. Here we describe the modular construction of a hydrogel using an engineered calmodulin (CaM) within a polyethylene glycol (PEG) matrix that involves the reversible tethering of proteins through an engineered CaM-binding sequence. For these measurements, maltose binding protein (MBP) was isotopically labeled with [13C] and [15N], permitting dynamic structural measurements using TROSY-HSQC NMR spectroscopy. Upon initial formation of hydrogels protein dynamics are suppressed, with concomitant increases in protein stability. Relaxation of the hydrogel matrix following transient heating results in the activation of protein dynamics and restoration of substrate-induced large-amplitude domain motions necessary for substrate binding.

Authors:: Beech, Brenda M.; Xiong, Yijia; Boschek, Curt B.; Baird, Cheryl L.; Bigelow, Diana J.; Mcateer, Kathleen; Squier, Thomas C.

Publication Date:: Fri Sep 05 00:00:00 EDT 2014

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1168881

Report Number(s):: PNNL-SA-100145
400403909

DOE Contract Number:: AC05-76RL01830

Resource Type:: Journal Article

Journal Name:: Journal of the American Chemical Society, 136(38):13134-13137

Additional Journal Information:: Journal Name: Journal of the American Chemical Society, 136(38):13134-13137

Country of Publication:: United States

Language:: English

Citation Formats


                    Beech, Brenda M., Xiong, Yijia, Boschek, Curt B., Baird, Cheryl L., Bigelow, Diana J., Mcateer, Kathleen, and Squier, Thomas C. Controlled Activation of Protein Rotational Dynamics Using Smart Hydrogel Tethering.  United States: N. p., 2014. 
        Web.  doi:10.1021/ja506717v.

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                    Beech, Brenda M., Xiong, Yijia, Boschek, Curt B., Baird, Cheryl L., Bigelow, Diana J., Mcateer, Kathleen, & Squier, Thomas C. Controlled Activation of Protein Rotational Dynamics Using Smart Hydrogel Tethering.  United States.  https://doi.org/10.1021/ja506717v

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                    Beech, Brenda M., Xiong, Yijia, Boschek, Curt B., Baird, Cheryl L., Bigelow, Diana J., Mcateer, Kathleen, and Squier, Thomas C. 2014.  
        "Controlled Activation of Protein Rotational Dynamics Using Smart Hydrogel Tethering".  United States.  https://doi.org/10.1021/ja506717v.

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@article{osti_1168881,

  title        = {Controlled Activation of Protein Rotational Dynamics Using Smart Hydrogel Tethering},

  author       = {Beech, Brenda M. and Xiong, Yijia and Boschek, Curt B. and Baird, Cheryl L. and Bigelow, Diana J. and Mcateer, Kathleen and Squier, Thomas C.},

  abstractNote = {Stimulus-responsive hydrogel materials that stabilize and control protein dynamics have the potential to enable a range of applications to take advantage of the inherent specificity and catalytic efficiencies of proteins. Here we describe the modular construction of a hydrogel using an engineered calmodulin (CaM) within a polyethylene glycol (PEG) matrix that involves the reversible tethering of proteins through an engineered CaM-binding sequence. For these measurements, maltose binding protein (MBP) was isotopically labeled with [13C] and [15N], permitting dynamic structural measurements using TROSY-HSQC NMR spectroscopy. Upon initial formation of hydrogels protein dynamics are suppressed, with concomitant increases in protein stability. Relaxation of the hydrogel matrix following transient heating results in the activation of protein dynamics and restoration of substrate-induced large-amplitude domain motions necessary for substrate binding.},

  doi          = {10.1021/ja506717v},

  url          = {https://www.osti.gov/biblio/1168881},
  journal      = {Journal of the American Chemical Society, 136(38):13134-13137},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Fri Sep 05 00:00:00 EDT 2014},

  month        = {Fri Sep 05 00:00:00 EDT 2014}

}

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