Hierarchically Ordered Nanopatterns for Spatial Control of Biomolecules

Tran, Helen; Ronaldson, Kacey; Bailey, Nevette A.; Lynd, Nathaniel A.; Killops, Kato L.; Vunjak-Novakovic, Gordana; Campos, Luis M.

doi:10.1021/nn505548n

Title: Hierarchically Ordered Nanopatterns for Spatial Control of Biomolecules

Journal Article · Tue Nov 04 00:00:00 EST 2014 · ACS Nano

DOI:https://doi.org/10.1021/nn505548n· OSTI ID:1168843

Tran, Helen ^[1]; Ronaldson, Kacey ^[2]; Bailey, Nevette A. ^[1]; Lynd, Nathaniel A. ^[3]; Killops, Kato L. ^[4]; Vunjak-Novakovic, Gordana ^[2]; Campos, Luis M. ^[1]

Columbia Univ., New York, NY (United States). Dept. of Chemistry
Columbia Univ., New York, NY (United States). Dept. of Biomedical Engineering
Univ. of Texas, Austin, TX (United States). McKetta Dept. of Chemical Engineering
Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD (United States)

The development and study of a benchtop, high-throughput, and inexpensive fabrication strategy to obtain hierarchical patterns of biomolecules with sub-50 nm resolution is presented. A diblock copolymer of polystyrene-b-poly(ethylene oxide), PS-b-PEO, is synthesized with biotin capping the PEO block and 4-bromostyrene copolymerized within the polystyrene block at 5 wt %. These two handles allow thin films of the block copolymer to be postfunctionalized with biotinylated biomolecules of interest and to obtain micropatterns of nanoscale-ordered films via photolithography. The design of this single polymer further allows access to two distinct superficial nanopatterns (lines and dots), where the PEO cylinders are oriented parallel or perpendicular to the substrate. Moreover, we present a strategy to obtain hierarchical mixed morphologies: a thin-film coating of cylinders both parallel and perpendicular to the substrate can be obtained by tuning the solvent annealing and irradiation conditions.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF); Department of Defense (DoD)

Grant/Contract Number:: AC02-06CH11357; AC02-05CH11231

OSTI ID:: 1168843

Alternate ID(s):: OSTI ID: 1511388

Journal Information:: ACS Nano, Vol. 8, Issue 11; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 20 works

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