skip to main content

Title: Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.
Authors:
Publication Date:
OSTI Identifier:
1167243
Report Number(s):
8,932,576
12/695,101
DOE Contract Number:
FG03-02ER15313
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Jan 27
Research Org:
The Scripps Research Institute, La Jolla, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES