Low-dose gold nanoparticles exert subtle endocrine-modulating effects on the ovarian steroidogenic pathway ex vivo independent of oxidative stress
Abstract
Gold nanoparticles (GNPs) have gained considerable attention for application in science and industry. However, the untoward effects of such particles on female fertility remain unclear. The objectives of this study were to (1) examine the effects of 10-nm GNPs on progesterone and estradiol-17b accumulation by rat ovaries ex vivo and (2) to identify the locus/loci whereby GNPs modulate steroidogenesis via multiple-reference gene quantitative real-time RT-PCR. Regression analyses indicated a positive relationship between both Star (p < 0.05, r2 = 0.278) and Cyp11a1 (p < 0.001, r2 = 0.366) expression and P4 accumulation. upon exposure to 1.43 * 106 GNPs/mL. Additional analyses showed that E2 accumulation was positively associated with Hsd3b1 (p < 0.05, r2 = 0.181) and Cyp17a1 (p < 0.01, r2 = 0.301) expression upon exposure to 1.43 * 13 and 1.43 * 109 GNPs/mL, respectively. These results suggest a subtle treatmentdependent impact of low-dose GNPs on the relationship between progesterone or estradiol-17b and specific steroidogenic target genes, independent of oxidative stress or inhibin.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1166850
- Report Number(s):
- PNNL-SA-101177
400412000
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Journal Article
- Journal Name:
- Nanotoxicology, 8(8):856-66
- Additional Journal Information:
- Journal Name: Nanotoxicology, 8(8):856-66
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Larson, Jeremy K., Carvan, Michael J., Teeguarden, Justin G., Watanabe, Gen, Taya, Kazuyoshi, Krystofiak, Evan, and Hutz, Reinhold J. Low-dose gold nanoparticles exert subtle endocrine-modulating effects on the ovarian steroidogenic pathway ex vivo independent of oxidative stress. United States: N. p., 2014.
Web. doi:10.3109/17435390.2013.837208.
Larson, Jeremy K., Carvan, Michael J., Teeguarden, Justin G., Watanabe, Gen, Taya, Kazuyoshi, Krystofiak, Evan, & Hutz, Reinhold J. Low-dose gold nanoparticles exert subtle endocrine-modulating effects on the ovarian steroidogenic pathway ex vivo independent of oxidative stress. United States. https://doi.org/10.3109/17435390.2013.837208
Larson, Jeremy K., Carvan, Michael J., Teeguarden, Justin G., Watanabe, Gen, Taya, Kazuyoshi, Krystofiak, Evan, and Hutz, Reinhold J. 2014.
"Low-dose gold nanoparticles exert subtle endocrine-modulating effects on the ovarian steroidogenic pathway ex vivo independent of oxidative stress". United States. https://doi.org/10.3109/17435390.2013.837208.
@article{osti_1166850,
title = {Low-dose gold nanoparticles exert subtle endocrine-modulating effects on the ovarian steroidogenic pathway ex vivo independent of oxidative stress},
author = {Larson, Jeremy K. and Carvan, Michael J. and Teeguarden, Justin G. and Watanabe, Gen and Taya, Kazuyoshi and Krystofiak, Evan and Hutz, Reinhold J.},
abstractNote = {Gold nanoparticles (GNPs) have gained considerable attention for application in science and industry. However, the untoward effects of such particles on female fertility remain unclear. The objectives of this study were to (1) examine the effects of 10-nm GNPs on progesterone and estradiol-17b accumulation by rat ovaries ex vivo and (2) to identify the locus/loci whereby GNPs modulate steroidogenesis via multiple-reference gene quantitative real-time RT-PCR. Regression analyses indicated a positive relationship between both Star (p < 0.05, r2 = 0.278) and Cyp11a1 (p < 0.001, r2 = 0.366) expression and P4 accumulation. upon exposure to 1.43 * 106 GNPs/mL. Additional analyses showed that E2 accumulation was positively associated with Hsd3b1 (p < 0.05, r2 = 0.181) and Cyp17a1 (p < 0.01, r2 = 0.301) expression upon exposure to 1.43 * 13 and 1.43 * 109 GNPs/mL, respectively. These results suggest a subtle treatmentdependent impact of low-dose GNPs on the relationship between progesterone or estradiol-17b and specific steroidogenic target genes, independent of oxidative stress or inhibin.},
doi = {10.3109/17435390.2013.837208},
url = {https://www.osti.gov/biblio/1166850},
journal = {Nanotoxicology, 8(8):856-66},
number = ,
volume = ,
place = {United States},
year = {Mon Dec 01 00:00:00 EST 2014},
month = {Mon Dec 01 00:00:00 EST 2014}
}