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Title: MALDI-MS analysis and imaging of small molecule metabolites with 1,5-diaminonaphthalene (DAN)

1,5-diaminonaphthalene (DAN) has previously been reported as an effective matrix for matrix-assisted laser desorption ionization-mass spectrometry of phospholipids. In the current work, we investigate the use of DAN as a matrix for small metabolite analysis in negative ion mode. DAN was found to provide superior ionization to the compared matrices for MW < ~400 Da; however, 9-aminoacridine (9-AA) was found to be superior for a uridine diphosphate standard (MW 566 Da). DAN was also found to provide a more representative profile of a natural phospholipid mixture than 9-AA. Finally, DAN and 9-AA were applied for imaging of metabolites directly from corn leaf sections. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.
 [1] ;  [1]
  1. Ames Laboratory
Publication Date:
OSTI Identifier:
Report Number(s):
IS-J 8448
Journal ID: ISSN 1076-5174
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Mass Spectrometry; Journal Volume: 49; Journal Issue: 8
Research Org:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
36 MATERIALS SCIENCE; MALDI; mass spectrometry imaging; matrix; metabolites; small molecules; 1,5-diaminonaphthalene