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Title: High temperature pre-digestion of corn stover biomass for improved product yields

Journal Article · · Biotechnology for Biofuels
 [1];  [1];  [1];  [2];  [2];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). Chemical Biosciences Center.
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center.
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Introduction: The efficient conversion of lignocellulosic feedstocks remains a key step in the commercialization of biofuels. One of the barriers to cost-effective conversion of lignocellulosic biomass to sugars remains the enzymatic saccharification process step. Here, we describe a novel hybrid processing approach comprising enzymatic pre-digestion with newly characterized hyperthermophilic enzyme cocktails followed by conventional saccharification with commercial enzyme preparations. Dilute acid pretreated corn stover was subjected to this new procedure to test its efficacy. Thermal tolerant enzymes from Acidothermus cellulolyticus and Caldicellulosiruptor bescii were used to pre-digest pretreated biomass at elevated temperatures prior to saccharification by the commercial cellulase formulation. Results: We report that pre-digestion of biomass with these enzymes at elevated temperatures prior to addition of the commercial cellulase formulation increased conversion rates and yields when compared to commercial cellulase formulation alone under low solids conditions. In conclusion, Our results demonstrating improvements in rates and yields of conversion point the way forward for hybrid biomass conversion schemes utilizing catalytic amounts of hyperthermophilic enzymes.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1166653
Report Number(s):
NREL/JA-2700-62534; MainId:24397; UUID:f6a9aa36-d51c-e411-b769-d89d67132a6d; MainAdminID:12873
Journal Information:
Biotechnology for Biofuels, Vol. 7, Issue 1; ISSN 1754-6834
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

References (15)

Technoeconomic analysis of the dilute sulfuric acid and enzymatic hydrolysis process for the conversion of corn stover to ethanol journal June 2009
The challenge of enzyme cost in the production of lignocellulosic biofuels journal November 2011
Modelling ethanol production from cellulose: separate hydrolysis and fermentation versus simultaneous saccharification and fermentation journal January 2009
A short review on SSF – an interesting process option for ethanol production from lignocellulosic feedstocks journal January 2008
Pretreatment Methods for Bioethanol Production journal June 2014
Comparison of SHF and SSF processes for the bioconversion of steam-exploded wheat straw journal October 2000
Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA journal December 2013
A new thermostable endoglucanase,Acidothermus cellulolyticus E1: Synergism withTrichoderma reesei CBH I and comparison to Thermomonospora fusca E5 journal March 1994
Properties and gene structure of a bifunctional cellulolytic enzyme (CelA) from the extreme thermophile 'Anaerocellum thermophilum' with separate glycosyl hydrolase family 9 and 48 catalytic domains journal February 1998
Crystal Structure of Thermostable Family 5 Endocellulase E1 from Acidothermus cellulolyticus in Complex with Cellotetraose † , ‡ journal January 1996
Purification and properties of recombinant ?-glucosidase of the hyperthermophilic bacterium Thermotoga maritima journal October 1993
Substrate specificity and transglycosylation catalyzed by a thermostable β-glucosidase from marine hyperthermophile Thermotoga neapolitana journal August 2005
Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover report June 2002
Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover report March 2011
New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences journal January 2021

Cited By (3)

Galactomannan degradation by thermophilic enzymes: a hot topic for biotechnological applications journal January 2019
Characterization of hemicellulase and cellulase from the extremely thermophilic bacterium Caldicellulosiruptor owensensis and their potential application for bioconversion of lignocellulosic biomass without pretreatment journal August 2015
Biochemical characterization of extra- and intracellular endoxylanse from thermophilic bacterium Caldicellulosiruptor kronotskyensis journal February 2016

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Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
biomass
pretreatment
enzymatic hydrolysis
CelA
E1
Caldicellulosiruptor bescii
Acidothermus cellulolyticus
Thermotoga maritima