U.S. Department of EnergyOffice of Scientific and Technical Information
Pnp gene modification for improved xylose utilization in Zymomonas
Abstract
The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.
- Inventors:
- Publication Date:
- Research Org.:
- E I Du Pont de Nemours and Company, Wilmington, DE (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1165440
- Patent Number(s):
- 8,911,983
- Application Number:
- 13/711,646
- Assignee:
- E I Du Pont de Nemours and Company (Wilmington, DE)
- DOE Contract Number:
- FC36-07GO17056
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Dec 12
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS
Citation Formats
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. Pnp gene modification for improved xylose utilization in Zymomonas. United States: N. p., 2014.
Web.
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, & Yang, Jianjun. Pnp gene modification for improved xylose utilization in Zymomonas. United States.
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. 2014.
"Pnp gene modification for improved xylose utilization in Zymomonas". United States. https://www.osti.gov/servlets/purl/1165440.
@article{osti_1165440,
title = {Pnp gene modification for improved xylose utilization in Zymomonas},
author = {Caimi, Perry G G and Qi, Min and Tao, Luan and Viitanen, Paul V and Yang, Jianjun},
abstractNote = {The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.},
doi = {},
url = {https://www.osti.gov/biblio/1165440},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 16 00:00:00 EST 2014},
month = {Tue Dec 16 00:00:00 EST 2014}
}
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Works referencing / citing this record:
Enhancing D-xylose and L-arabinose utilization in Zymomonas cells
patent, June 2016
- Eliot, Andrew C.; Tao, Luan; Viitanen, Paul V.
- US Patent Document 9,371,546