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Title: Isolation, cultivation and genomic analysis of magnetosome biomineralization genes of a new genus of South-seeking magnetotactic cocci within the Alphaproteobacteria

Although magnetotactic bacteria (MTB) are ubiquitous in aquatic habitats, they are still considered fastidious microorganisms with regard to growth and cultivation with only a relatively low number of axenic cultures available to date. Here, we report the first axenic culture of an MTB isolated in the Southern Hemisphere (Itaipu Lagoon in Rio de Janeiro, Brazil). Cells of this new isolate are coccoid to ovoid in morphology and grow microaerophilically in semi-solid medium containing an oxygen concentration ([O2]) gradient either under chemoorganoheterotrophic or chemolithoautotrophic conditions. Each cell contains a single chain of approximately 10 elongated cuboctahedral magnetite (Fe3O4) magnetosomes. Phylogenetic analysis based on the 16S rRNA gene sequence shows that the coccoid MTB isolated in this study represents a new genus in the Alphaproteobacteria; the name Magnetofaba australis strain IT-1 is proposed. Preliminary genomic data obtained by pyrosequencing shows that M. australis strain IT-1 contains a genomic region with genes involved in biomineralization similar to those found in the most closely related magnetotactic cocci Magnetococcus marinus strain MC-1. However, organization of the magnetosome genes differs from M. marinus.
 [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [2] ;  [3] ;  [1]
  1. Universidade Federal do Rio de Janeiro
  2. Laboratorio Nacional de Computacao Cientifica
  3. Ames Laboratory
Publication Date:
OSTI Identifier:
Report Number(s):
IS-j 8445
Journal ID: ISSN 1664-302X
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Frontiers in Microbiology; Journal Volume: 5
Research Org:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
36 MATERIALS SCIENCE; Magnetofaba australis strain IT-1, magnetite, magnetosome, South-seeking magnetotactic bacteria, biomineralization genes