High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery
Journal Article
·
· Angewandte Chemie (International Edition)
A high-throughput protein-expression and screening method (HIGH method, see picture) provides a rapid approach to the discovery of active glycoside hydrolases in environmental samples. Finally, HIGH screening combines cloning, protein expression, and enzyme hydrolysis in one pot; thus, the entire process from gene expression to activity detection requires only three hours.
- Research Organization:
- USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI ID:
- 1153466
- Journal Information:
- Angewandte Chemie (International Edition), Vol. 50, Issue 47; ISSN 1433-7851
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
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