Single-Molecule Study of Protein-Protein Interaction Dynamics in a Cell Signaling System
We report a study on protein-protein noncovalent interactions in an intracellular signaling protein complex, using single-molecule spectroscopy and molecular dynamics (MD) simulations. A Wiskott-Aldrich Syndrome Protein (WASP) fragment that binds only the activated intracellular signaling protein Cdc42 was labeled with a novel solvatochromic dye and used to probe hydrophobic interactions significant to Cdc42/WASP recognition. The study shows static and dynamic inhomogeneous conformational fluctuations of the protein complex that involve bound and loosely bound states. A two-coupled, two-state Markovian kinetic model is proposed for the conformational dynamics. Finally, the MD simulations explore the origin of these conformational states and associated conformational fluctuations in this protein-protein interaction system.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI ID:
- 1152392
- Journal Information:
- Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry, Vol. 108, Issue 2; ISSN 1520-6106
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
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