The mutagenicity of food mutagenes in the intestine
- York Univ. Ontario (Canada)
- Lawrence Livermore National Laboratory, CA (United States); and others
PhIP and amino ({alpha})carboline (AAC) have been identified as mutagens in cooked food by means of the Ames test. In this study they were incorporated in the diet (400 and 800 ppm respectively) of groups of 3 {male} {male} and 3 {female} {female} mice F{sub 1} (C57B1/6 X SWR) mice heterozygous for a lac1 transgene (the Big Blue {trademark} Mouse) and at Dlb-1. The Dlb-1 locus controls the presence (+) or absence of a lectin-binding site in the small intestine so mutational loss of the dominant + allele can be seen as ribbons of non-staining cells on the villi. PhIP has been shown to be mutagenic in the small intestine by Brooks et al., with this assay. Its activity in the colon, where human cancers arise, has not been reported. lac1 mutations were assayed in the epithelial cells of both the colon and small intestine. PhIP was mutagenic in the Dlb-1 assay (thereby confirming Brooks et al.) and induced lac1 mutations in both colon and small intestine about equally. AAC was not mutagenic in the small intestine at Dlb-1 or lac1, but induced lac1 mutations at a rate (mutations/ppm*days) in the colon similar to PhIP. The concentrations of PhIP and AAC were about 10,000 times that found in the human diet, but the maximum exposures were much shorter: 90 and 45 days respectively.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 115155
- Report Number(s):
- CONF-9503160-; ISSN 0893-6692; CNN: Grant CA55861-01; TRN: 95:004640-0050
- Journal Information:
- Environmental and Molecular Mutagenesis, Vol. 25, Issue Suppl.25; Conference: 26. annual Environmental Mutagen Society meeting, St. Louis, MO (United States), 12-16 Mar 1995; Other Information: PBD: 1995
- Country of Publication:
- United States
- Language:
- English
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