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Title: Systems level insights into alternate methane cycling modes in a freshwater lake via community transcriptomics, metabolomics and nano-SIMS analysis

The research conducted as part of this project contributes significantly to the understanding of the microbes and their activities involved in methane metabolism in freshwater lake sediments and in the environment in a more global sense. Significant new insights have been gained into the identity of the species that are most active in methane oxidation. New concepts have been developed based on the new data on how these organisms metabolize methane, impacting not only environmental microbiology but also biotechnology, including biotechnology of next generation biofuels. Novel approaches have been developed for studying functional microbial communities, via holistic approaches, such as metagenomics, metatrancriptomics and metabolite analysis. As a result, a novel outlook has been obtained at how such communities operate in nature. Understanding methane-oxidizing communities in lakes and other environments is of significant benefit to the public, in terms of methane emission mitigation and in terms of potential biotechnological applications.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [1]
  1. Univ. of Washington, Seattle, WA (United States)
  2. California Inst. of Technology (CalTech), Pasadena, CA (United States)
Publication Date:
OSTI Identifier:
1149958
Report Number(s):
DOE-UW--05154
DOE Contract Number:
SC0005154
Resource Type:
Technical Report
Research Org:
Univ. of Washington, Seattle, WA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Contributing Orgs:
California Institute of Technology, Qingdao Agricultural University, University of California-San Diego
Country of Publication:
United States
Language:
English
Subject:
03 NATURAL GAS; 54 ENVIRONMENTAL SCIENCES Final Report