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Title: From surface to intracellular non-invasive nanoscale study of living cells impairments

Among the enduring challenges in nanoscience, subsurface characterization of live cells holds major stakes. Developments in nanometrology for soft matter thriving on the sensitivity and high resolution benefits of atomic force microscopy have enabled detection of subsurface structures at the nanoscale (1,2,3). However, measurements in liquid environments remain complex (4,5,6,7), in particular in the subsurface domain. Here we introduce liquid-Mode Synthesizing Atomic Force Microscopy (l-MSAFM) to study both the inner structures and the chemically induced intracellular impairments of living cells. Specifically, we visualize the intracellular stress effects of glyphosate on living keratinocytes skin cells. This new approach for living cell nanoscale imaging, l-MSAFM, in their physiological environment or in presence of a chemical stress agent confirmed the loss of inner structures induced by glyphosate. The ability to monitor the cell's inner response to external stimuli, non-destructively and in real time, has the potential to unveil critical nanoscale mechanisms of life science.
 [1] ;  [2] ;  [3] ;  [4] ;  [2] ;  [1] ;  [1]
  1. University of Bourgogne, 21078 Dijon, France.
  2. ORNL
  3. Institut FEMTO-ST UMR CNRS 6174, University Franche-Comté, 25044 Besancon, France
  4. University of Franche-Comte, Laboratoire de Biologie Cellulaire
Publication Date:
OSTI Identifier:
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Nanotechnology; Journal Volume: 25; Journal Issue: 29
Research Org:
Oak Ridge National Laboratory (ORNL)
Sponsoring Org:
ORNL LDRD Director's R&D
Country of Publication:
United States