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Title: Recombinant clostridia that fix CO2 and CO and uses thereof

Patent ·
OSTI ID:1135908
; ; ; ; ;

The present invention relates a recombinant Clostridium expressing one or more heterologous Wood-Ljungdahl (WL) genes. In particular, the recombinant Clostridium produces a metabolite at an increased level. The present invention also relates to a method for producing a metabolite by the recombinant Clostridium.

Research Organization:
University of Delaware, Newark, DE (USA).
Sponsoring Organization:
USDOE
Assignee:
University of Delaware (Newark, DE)
Patent Number(s):
8,759,070
Application Number:
13/229,033
OSTI ID:
1135908
Country of Publication:
United States
Language:
English

References (53)

Biological production of acetic acid from waste gases with Clostridium ljungdahlii patent September 1998
Biological production of products from waste gases patent January 2002
Methods and Organisms for Production of 3-Hydroxypropionic Acid patent-application January 2010
Methods and Composition for Generating Sporulation Deficient Bacteria patent-application February 2010
Process for the Biological Production of n-Butanol with High Yield patent-application April 2010
Process for the Biological Production of 1.3-Propanediol from Glycerol with High Yield patent-application June 2010
Transformant Capable of Producing Isopropanol patent-application August 2010
Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production. journal September 1994
Basic local alignment search tool journal October 1990
Systems-Level Metabolic Flux Profiling Elucidates a Complete, Bifurcated Tricarboxylic Acid Cycle in Clostridium acetobutylicum journal July 2010
Clostridium perfringens-Escherichia coli Shuttle Vectors That Carry Single Antibiotic Resistance Determinants journal May 1993
Distribution of Methanol Carbon Between Assimilation and Oxidation Pathways in Methanol-grown Pseudomonas C journal January 1980
Evidence That Ferredoxin Interfaces with an Internal Redox Shuttle in Acetyl-CoA Synthase during Reductive Activation and Catalysis journal January 2011
Natural transfer of conjugative transposon Tn916 between gram-positive and gram-negative bacteria. journal January 1991
A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing journal May 2010
The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain. journal September 1997
Physiology of the thermophilic acetogen Moorella thermoacetica journal December 2004
Bioproduction of butanol from biomass: from genes to bioreactors journal June 2007
Effect of nitrate on the autotrophic metabolism of the acetogens Clostridium thermoautotrophicum and Clostridium thermoaceticum. journal August 1996
Identification and molecular genetic analysis of replication functions of the bacteriocinogenic plasmid pIP404 from Clostridium perfringens journal March 1988
Metabolic pathways of clostridia for producing butanol journal November 2009
Fermentation characterization and flux analysis of recombinant strains of Clostridium acetobutylicum with an inactivated solR gene journal November 2001
Characterization of recombinant strains of the Clostridium acetobutylicum butyrate kinase inactivation mutant: Need for new phenomenological models for solventogenesis and butanol inhibition? journal January 2000
Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression in Clostridium perfringens journal November 2010
A modular system for Clostridium shuttle plasmids journal July 2009
PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum journal May 2008
Purification and Characterization of Membrane-associated CooC Protein and Its Functional Role in the Insertion of Nickel into Carbon Monoxide Dehydrogenase from Rhodospirillum rubrum journal August 2001
The transcriptional program underlying the physiology of clostridial sporulation journal January 2008
Inactivation of  F in Clostridium acetobutylicum ATCC 824 Blocks Sporulation Prior to Asymmetric Division and Abolishes  E and  G Protein Expression but Does Not Block Solvent Formation journal March 2011
KEGG: Kyoto Encyclopedia of Genes and Genomes journal January 2000
Clostridium ljungdahlii represents a microbial production platform based on syngas journal July 2010
Gas chromatography and gateway sensors for on-line state estimation of complex fermentations (butanol-acetone fermentation) journal August 1985
Expression of Cloned Homologous Fermentative Genes in Clostridium Acetobutylicum ATCC 824 journal February 1992
Sequence and properties of pIM13, a macrolide-lincosamide-streptogramin B resistance plasmid from Bacillus subtilis. journal July 1986
Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum journal August 2001
Engineering solventogenic clostridia journal October 2008
The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum) journal July 2008
Replication properties of pIM13, a naturally occurring plasmid found in Bacillus subtilis, and of its close relative pE5, a plasmid native to Staphylococcus aureus. journal November 1987
The Eastern and Western branches of the Wood/Ljungdahl pathway: how the East and West were won journal January 1997
Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation journal December 2008
Ethanol production from H 2 and CO 2 by a newly isolated thermophilic bacterium, Moorella sp. HUC22-1 journal October 2004
Acetate and ethanol production from H2 and CO2by Moorella sp. using a repeated batch culture journal March 2005
Optimization of a heterologous pathway for the production of flavonoids from glucose journal July 2011
Isolation of mutants ofClostridium acetobutylicum ATCC 824 deficient in protease activity journal March 1993
The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome journal June 2006
Nitrate as a preferred electron sink for the acetogen Clostridium thermoaceticum. journal December 1993
Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance journal November 2008
Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations journal January 2009
Analysis of Degenerate Variants ofClostridium acetobutylicumATCC 824 journal February 1996
DNA Array-Based Transcriptional Analysis of Asporogenous, Nonsolventogenic Clostridium acetobutylicum Strains SKO1 and M5 journal July 2003
Inactivation of  E and  G in Clostridium acetobutylicum Illuminates Their Roles in Clostridial-Cell-Form Biogenesis, Granulose Synthesis, Solventogenesis, and Spore Morphogenesis journal January 2011
Genetic Methods in Clostridia book January 1999
mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis journal January 2006

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Recombinant clostridia that fix CO2 and CO and uses thereof patent June 2014

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